Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 33331..33878 Replicon plasmid p2461-2
Accession NZ_CP095534
Organism Escherichia coli strain GN02461

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag MW400_RS24110 Protein ID WP_001384452.1
Coordinates 33600..33878 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag MW400_RS24105 Protein ID WP_000079928.1
Coordinates 33331..33600 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW400_RS24080 (MW400_24070) 28975..29508 - 534 WP_001303319.1 transcription termination/antitermination NusG family protein -
MW400_RS24085 (MW400_24075) 29950..30237 - 288 WP_000074849.1 conjugal transfer protein TraA -
MW400_RS24090 (MW400_24080) 30155..30433 - 279 WP_219065241.1 ash family protein -
MW400_RS24095 (MW400_24085) 31391..32422 + 1032 WP_000907874.1 plasmid replication initiator RepA -
MW400_RS24100 (MW400_24090) 33118..33276 + 159 WP_000841008.1 hypothetical protein -
MW400_RS24105 (MW400_24095) 33331..33600 + 270 WP_000079928.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
MW400_RS24110 (MW400_24100) 33600..33878 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
MW400_RS24115 (MW400_24105) 33918..34766 + 849 WP_001057996.1 3'-5' exonuclease -
MW400_RS24120 (MW400_24110) 34883..35149 + 267 Protein_36 hypothetical protein -
MW400_RS24125 (MW400_24115) 35321..36520 - 1200 WP_000948429.1 IS91 family transposase -
MW400_RS24130 (MW400_24120) 36530..36718 - 189 WP_000957857.1 hypothetical protein -
MW400_RS24135 (MW400_24125) 37115..38260 + 1146 WP_000976514.1 class C beta-lactamase CMY-2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCMY-2 - 1..78130 78130
flank IS/Tn blaCMY-2 - 35321..38260 2939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T242346 WP_001384452.1 NZ_CP095534:33600-33878 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T242346 NZ_OW052570:2870832-2871224 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT242346 WP_000079928.1 NZ_CP095534:33331-33600 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT242346 NZ_OW052570:2870557-2870832 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References