6114

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RelE
Location 29150..29673 Replicon metagenomic gene cluster
Accession KF033132
Organism uncultured bacterium clone 93

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag Locus_27 Protein ID AGT60063.1
Coordinates 29150..29443 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag Locus_28 Protein ID AGT60064.1
Coordinates 29434..29673 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Locus_21 24191..25258 - 1068 AGT60057.1 ribosome-associated GTPase -
Locus_22 25285..25926 - 642 AGT60058.1 hypothetical protein -
Locus_23 26066..26176 - 111 AGT60059.1 hypothetical protein -
Locus_24 26189..26875 - 687 AGT60060.1 Fic/Doc protein -
Locus_25 26880..27686 - 807 AGT60061.1 hypothetical protein -
Locus_26 27701..29062 - 1362 AGT60062.1 hypothetical protein -
Locus_27 29150..29443 - 294 AGT60063.1 RelE/StbE Toxin
Locus_28 29434..29673 - 240 AGT60064.1 CopG Antitoxin
Locus_29 29837..30721 - 885 AGT60065.1 HisG -
Locus_30 30759..31544 - 786 AGT60066.1 HisB -
Locus_31 31563..32192 - 630 AGT60067.1 HisI -
Locus_32 32262..33029 - 768 AGT60068.1 HisF -
Locus_33 33370..34317 + 948 AGT60069.1 TehA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 11411.55 Da        Isoelectric Point: 10.2958

>T6114 AGT60063.1 KF033132:c29443-29150 [uncultured bacterium]
MALNILWTKRVLANLESELDYYGQINLLLAKELTLIIRQSIDTIYRMPAIGRAGKKIGTRELVIEKYPYILAYRVRNDVL
EILAIIHQKRKNIRSHY

Download         Length: 294 bp

>T6114 KF033132:c29443-29150 [uncultured bacterium]
ATGGCTCTAAATATCCTGTGGACGAAACGCGTACTAGCCAACTTAGAGTCTGAGCTTGATTACTATGGACAAATTAATCT
ACTCTTGGCAAAAGAACTAACTTTAATCATTCGACAATCTATCGACACTATTTATCGTATGCCAGCCATTGGACGCGCAG
GTAAGAAGATTGGCACTCGGGAGTTAGTCATTGAAAAATACCCATATATTTTGGCATATCGCGTGAGAAATGATGTTCTT
GAAATTTTGGCAATAATTCATCAAAAGCGCAAGAATATCCGCAGTCATTATTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9103.42 Da        Isoelectric Point: 4.5173

>AT6114 AGT60064.1 KF033132:c29673-29434 [uncultured bacterium]
MKTENPVISFRVPSEIYDKLEQLVKATKRNKADLLLHWLEEAISLEEWQLQEVEAGIKEADAGMFASSAETERVLTKWL

Download         Length: 240 bp

>AT6114 KF033132:c29673-29434 [uncultured bacterium]
ATGAAAACAGAAAACCCCGTAATTTCATTTCGCGTACCAAGTGAAATATATGATAAGCTCGAACAACTAGTTAAAGCGAC
TAAGCGCAACAAGGCTGATTTGTTATTACATTGGCTAGAAGAAGCAATATCCCTCGAAGAGTGGCAACTACAGGAAGTTG
AAGCCGGAATTAAAGAAGCCGACGCAGGTATGTTTGCATCATCCGCAGAAACAGAGCGAGTTTTAACCAAATGGCTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10119 Escherichia coli

31.868

98.913

0.315

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References

(1) Ken Vercammen et al. (2013) Identification of a metagenomic gene cluster containing a new class A beta-lactamase and toxin-antitoxin systems. MicrobiologyOpen 2(4):674-83. [PubMed:23873667] experimental


experimental experimental literature