705

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) relBE/RHH-RelE
Location 1935447..1936075 Replicon chromosome
Accession NC_004603
Organism Vibrio parahaemolyticus RIMD 2210633

Toxin (Protein)


Gene name relE Uniprot ID Q87NM5
Locus tag VP1843 Protein ID WP_005483176.1
Coordinates 1935797..1936075 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0L8TUJ0
Locus tag VP1842 Protein ID WP_005483196.1
Coordinates 1935516..1935800 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VP_RS08890 1930888..1931355 - 468 WP_005464650.1 cold shock domain-containing protein -
VP_RS08895 1931456..1931533 - 78 WP_072833152.1 DUF3265 domain-containing protein -
VP_RS08900 (VP1835) 1931562..1931936 - 375 WP_005483170.1 hypothetical protein -
VP_RS08905 1932074..1932583 - 510 WP_005483274.1 ClbS/DfsB family four-helix bundle protein -
VP_RS08910 (VP1838) 1933192..1933284 - 93 WP_011105963.1 DUF3265 domain-containing protein -
VP_RS08915 (VP1839) 1933312..1933824 - 513 WP_024699234.1 hypothetical protein -
VP_RS08920 (VP1840) 1933966..1934547 - 582 WP_005483293.1 hypothetical protein -
VP_RS08925 1934575..1934697 - 123 WP_021451208.1 DUF3265 domain-containing protein -
VP_RS08930 (VP1841) 1934681..1935319 - 639 WP_005483241.1 hypothetical protein -
VP_RS08935 1935348..1935439 - 92 Protein_1739 DUF3265 domain-containing protein -
VP_RS08940 (VP1842) 1935516..1935800 + 285 WP_005483196.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
VP_RS08945 (VP1843) 1935797..1936075 + 279 WP_005483176.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
VP_RS08950 1936104..1936220 - 117 WP_080931826.1 DUF3265 domain-containing protein -
VP_RS08955 (VP1844) 1936227..1936748 - 522 WP_005483288.1 GNAT family N-acetyltransferase -
VP_RS08960 (VP1845) 1936887..1937561 - 675 WP_005483207.1 hypothetical protein -
VP_RS08965 (VP1847) 1937695..1938093 - 399 WP_005483218.1 DUF1311 domain-containing protein -
VP_RS08970 (VP1848) 1938305..1938979 - 675 WP_005483286.1 hypothetical protein -
VP_RS08975 1939126..1939398 - 273 WP_029853404.1 hypothetical protein -
VP_RS08980 (VP1849) 1939537..1939776 - 240 WP_005483258.1 hypothetical protein -
VP_RS08985 1939913..1940002 - 90 WP_072833189.1 DUF3265 domain-containing protein -
VP_RS08990 (VP1850) 1940031..1940447 - 417 WP_021451209.1 GNAT family N-acetyltransferase -
VP_RS08995 1940492..1940584 - 93 WP_072833185.1 DUF3265 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integron - - 1901751..1949243 47492


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(1-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-45)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10906.55 Da        Isoelectric Point: 4.3430

>T705 WP_005483176.1 NC_004603:1935797-1936075 [Vibrio parahaemolyticus RIMD 2210633]
MILWEEESLNDREEIFEFLYDFNPDAAEKTDNLIEAKVENLLKQPLMGVQRDGIRGRLLIIPEISMIVSYWVEGDIIRVM
RVQHQKQKFPTD

Download         Length: 279 bp

>T705 NC_004603:1935797-1936075 [Vibrio parahaemolyticus RIMD 2210633]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAGAGATCTTTGAGTTTCTCTATGACTTCAACCCTGATGCTGC
AGAAAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTAAACAACCTTTAATGGGTGTGCAACGAGATGGCA
TCCGCGGACGATTACTCATCATTCCTGAGATTTCGATGATTGTATCTTACTGGGTTGAGGGCGATATCATCCGAGTTATG
CGTGTACAACACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 11083.43 Da        Isoelectric Point: 9.2167

>AT705 WP_005483196.1 NC_004603:1935516-1935800 [Vibrio parahaemolyticus RIMD 2210633]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKTLSHDAWLTEQVNLAFEKFDSGKSVFLEHQTAKSR
MEERKARIRNRGKQ

Download         Length: 285 bp

>AT705 NC_004603:1935516-1935800 [Vibrio parahaemolyticus RIMD 2210633]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGCAC
TCTGAGTGACGCATGTCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTGTTTTCCTTGAGCACCAAACGGCTAAGTCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGAGGTAAGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T168 Vibrio cholerae O1 biovar El Tor str. N16961

84.783

100

0.848
T179 Vibrio cholerae O1 biovar El Tor str. N16961

84.783

100

0.848
T6097 Sinorhizobium meliloti 1021

36.264

98.913

0.359
T10139 Pseudomonas savastanoi

30.769

100

0.308

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT179 Vibrio cholerae O1 biovar El Tor str. N16961

90.217

97.872

0.883
AT168 Vibrio cholerae O1 biovar El Tor str. N16961

90.217

97.872

0.883

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB Q87NM5

Antitoxin

Source ID Structure
AlphaFold DB A0A0L8TUJ0

References

(1) Jing Zhang et al. (2017) Expression of a Vibrio parahaemolyticus toxin in Escherichia coli results in chromosomal DNA degradation. Bioscience, Biotechnology, And Biochemistry 81(10):1937-1940. [PubMed:28820009] experimental

(2) M Hino et al. (2014) Characterization of putative toxin/antitoxin systems in Vibrio parahaemolyticus. Journal of Applied Microbiology 117(1):185-95. [PubMed:24698443] experimental

(3) Jing Zhang et al. (2017) A RelE/ParE superfamily toxin in Vibrio parahaemolyticus has DNA nicking endonuclease activity. Biochemical And Biophysical Research Communications 489(1):29-34. [PubMed:28533087] experimental


experimental experimental literature