Detailed information of TA system TA006257

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(antitoxin)
• Location: 1564529..1565095
• Replicon: chromosome
• Accession: NC_011593
• Organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W6EX94
• Locus tag: BLON_RS07270
• Protein ID: WP_003831807.1
• Coordinates: 1564802..1565095 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BLON_RS07265
• Protein ID: WP_014484914.1
• Coordinates: 1564529..1564798 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLON_RS07240 (Blon_1393)	1560279..1560973	-	695	Protein_1415	response regulator transcription factor	-
BLON_RS07245 (Blon_1394)	1561099..1561614	+	516	WP_013140733.1	hypothetical protein	-
BLON_RS07250 (Blon_1395)	1561611..1562501	+	891	WP_014484913.1	hypothetical protein	-
BLON_RS07255 (Blon_1396)	1562498..1563178	+	681	WP_003829413.1	ABC transporter ATP-binding protein	-
BLON_RS07260 (Blon_1397)	1563175..1564344	+	1170	WP_012577732.1	ABC transporter permease	-
BLON_RS07265 (Blon_1398)	1564529..1564798	+	270	WP_014484914.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLON_RS07270 (Blon_1399)	1564802..1565095	+	294	WP_003831807.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BLON_RS13660	1565153..1565433	+	281	Protein_1422	hypothetical protein	-
BLON_RS07280 (Blon_1401)	1565764..1566315	+	552	WP_012577735.1	RNA polymerase sigma factor	-
BLON_RS07285 (Blon_1402)	1566349..1567176	+	828	WP_012577736.1	hypothetical protein	-
BLON_RS07290 (Blon_1403)	1567308..1567934	+	627	WP_012577737.1	hypothetical protein	-
BLON_RS07295 (Blon_1404)	1567931..1568713	+	783	WP_012577738.1	hypothetical protein	-
BLON_RS07300 (Blon_1405)	1568766..1569641	+	876	WP_003829425.1	ABC transporter ATP-binding protein	-
BLON_RS07305 (Blon_1406)	1569695..1570093	+	399	WP_003829426.1	superoxide dismutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_017219 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM Genomic island TA026378 1555334..1576692 0 View NZ_LR134354 Bifidobacterium longum subsp. infantis strain NCTC11817 Genomic island TA287797 1555338..1576696 0 View NZ_LR655210 Bifidobacterium longum subsp. infantis isolate B.longum_ssp_infantis_6 Genomic island TA288655 1507377..1535379 0.007 View NZ_LR698953 Bifidobacterium longum isolate MGYG-HGUT-01292 Genomic island TA288685 1555544..1576902 0	inside	Genomic island	-	-	1555544..1576902	21358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-87)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11218.98 Da
• Isoelectric Point: 9.7881

>T6257 WP_003831807.1 NC_011593:1564802-1565095 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
MLKPDYTAAFQRDIKKLKRKHTDLRPLKEVIRLVLEDTADSKEVLRRRHRAHTLTGDLNGVLECHIGNAGDWLLLWIRDD
GTAMFMRTGSHDELLGK

Nucleotide

• Length: 294 bp

>T6257 NC_011593:1564802-1565095 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
ATGCTCAAACCGGATTACACAGCGGCATTCCAGCGAGATATCAAGAAACTCAAACGCAAGCACACTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTTCTTGAGGACACGGCGGACTCCAAGGAAGTATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATTTAAACGGCGTATTGGAATGCCACATCGGCAATGCCGGCGACTGGCTGCTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTGGGCAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9902.25 Da
• Isoelectric Point: 4.7872

>AT6257 WP_014484914.1 NC_011593:1564529-1564798 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
VSMASVTVRVDADTKQKAANIAEDLGLDLSSVTRAFYKQIVRERRIPLNLSYTTVPPETMEALDEAKNRLADGKPRFNSA
DEMFDSLVI

Nucleotide

• Length: 270 bp

>AT6257 NC_011593:1564529-1564798 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATTGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAGGCGCATTCCCCTAAACCTTTCCTATACGA
CCGTGCCGCCTGAAACCATGGAGGCCCTTGACGAGGCGAAGAACAGGCTCGCCGACGGCAAACCTCGTTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGTAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6289	Bifidobacterium longum subsp. longum JDM301	100	100	1
T10190	Pseudomonas sp. strain ANT_J3	37.755	100	0.381
T28	Helicobacter pylori 26695	34.737	100	0.375
T10111	Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes	36.458	98.969	0.361
T10095	Clostridium difficile R20291	36.17	97.917	0.354
T10112	Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes	35.417	98.969	0.351
T665	Streptococcus mutans UA159	31.633	100	0.337
T6256	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	32.258	96.875	0.312
T6127	Helicobacter pylori 26695	28.866	100	0.311
T10070	Tetragenococcus halophilus NBRC 12172	29.897	100	0.309

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT6289	Bifidobacterium longum subsp. longum JDM301	96.629	100	0.966
AT10181	Aggregatibacter actinomycetemcomitans D11S 1	35.714	94.382	0.337
AT10136	Pseudomonas savastanoi	33.735	93.258	0.315

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: W6EX94

References

(1) Olga Averina et al. (2015) Functional analysis of the type II toxin-antitoxin systems of the MazEF and RelBE families in Bifidobacterium longum subsp. infantis ATCC 15697. Anaerobe 35(Pt B):59-67. [PubMed:26210151]


 experimental literature