215714

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 2648824..2649309 Replicon chromosome
Accession NZ_CP082961
Organism Lacticaseibacillus rhamnosus strain CAU 1365

Toxin (Protein)


Gene name yafQ Uniprot ID C2K0Y1
Locus tag K6087_RS12155 Protein ID WP_005691636.1
Coordinates 2648824..2649009 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A508YYT1
Locus tag K6087_RS12160 Protein ID WP_014571130.1
Coordinates 2649037..2649309 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6087_RS12135 (K6087_12110) 2643831..2644520 + 690 WP_005691628.1 rhomboid family intramembrane serine protease -
K6087_RS12140 (K6087_12115) 2645012..2645620 - 609 WP_005691630.1 hypothetical protein -
K6087_RS12145 (K6087_12120) 2646067..2646942 + 876 WP_025014074.1 Rgg/GadR/MutR family transcriptional regulator -
K6087_RS12150 (K6087_12125) 2647185..2648600 - 1416 WP_005691634.1 exonuclease domain-containing protein -
K6087_RS12155 (K6087_12130) 2648824..2649009 - 186 WP_005691636.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
K6087_RS12160 (K6087_12135) 2649037..2649309 - 273 WP_014571130.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
K6087_RS12165 (K6087_12140) 2649871..2650119 + 249 WP_005691639.1 hypothetical protein -
K6087_RS12170 (K6087_12145) 2650362..2650655 + 294 WP_005684269.1 metalloregulator ArsR/SmtB family transcription factor -
K6087_RS12175 (K6087_12150) 2650737..2651900 + 1164 WP_005691640.1 MFS transporter -
K6087_RS12180 (K6087_12155) 2652054..2652629 + 576 WP_014571131.1 DNA-3-methyladenine glycosylase I -
K6087_RS12185 (K6087_12160) 2652772..2653368 + 597 WP_005691643.1 IS607 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2643831..2655034 11203


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-57)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-65)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6781.86 Da        Isoelectric Point: 7.4698

>T215714 WP_005691636.1 NZ_CP082961:c2649009-2648824 [Lacticaseibacillus rhamnosus]
MQRQGHVKLASLHDHALKGAHSGERALRVAPDWLLVYKVDAEALILMLLATGTHRDTLNIE

Download         Length: 186 bp

>T215714 NZ_CP110921:c2808085-2807894 [Methylococcus sp. 16-5]
ATGATAAGGACCTTCGCCGAAAAAGCCACCGATGCCGTGTTTGCCGGCGAAAGAGTCCGCAAATACCCGGCCAAACTGCT
CCAAATGGCCCGGCGGATACTGGCTTGGCTGGACCGGGCCGAACGGATCGAAAGCTTGAAGGTTCCACCCGGCAACCGCA
TCGAAGCTCTGGGCGGCGATCGGAACGACTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10005.62 Da        Isoelectric Point: 9.5939

>AT215714 WP_014571130.1 NZ_CP082961:c2649309-2649037 [Lacticaseibacillus rhamnosus]
MTKKSRISVRIDTKTKERALHVLNSMGLDISSAINMYLKRIGDTGALPFTPPMSFVDQLQVAEADVKAGRIKSFKTVDAL
MKDLYSDVDD

Download         Length: 273 bp

>AT215714 NZ_CP110921:c2807815-2807459 [Methylococcus sp. 16-5]
ATGACCATCCGCATTGAAGAAATCAACGCCCTCGACTTCTGCGACGTGGCCGACCCCCACCTGGGAAAGCTTGCGCCCAT
CCATCCCGGTGAAATCCTGCGCGAAGAGTTCTTGAAGCCCTTAGGACTCAGCGTGAACGCCCTGGCCCAAGCGTTAAGAG
TGCCCGCCACCCGCATCCATGAAATCGTCAAAGAACGCAGAGGCATCACCCCGGACACCGCATTGCGCCTGGCCCGCTAC
TTCGGAGGAGACGCCCGGATGTGGCTGAACCTCCAGGCCAACTTTGACTTGAAGATGGCCGCTCGGAGCTCCTGCATCGA
GCGGGAAGTGATTCCGCGCGAATCGACCGCCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X2J6C9

Antitoxin

Source ID Structure
AlphaFold DB A0A508YYT1

References