222966

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 2432889..2433374 Replicon chromosome
Accession NZ_CP086326
Organism Lacticaseibacillus rhamnosus strain PMC203

Toxin (Protein)


Gene name yafQ Uniprot ID C2K0Y1
Locus tag LL671_RS11215 Protein ID WP_005691636.1
Coordinates 2433189..2433374 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A508YYT1
Locus tag LL671_RS11210 Protein ID WP_014571130.1
Coordinates 2432889..2433161 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL671_RS11185 (LL671_11190) 2428830..2429426 - 597 WP_005691643.1 IS607 family transposase -
LL671_RS11190 (LL671_11195) 2429569..2430144 - 576 WP_014571131.1 DNA-3-methyladenine glycosylase I -
LL671_RS11195 (LL671_11200) 2430298..2431461 - 1164 WP_005691640.1 MFS transporter -
LL671_RS11200 (LL671_11205) 2431543..2431836 - 294 WP_005684269.1 metalloregulator ArsR/SmtB family transcription factor -
LL671_RS11205 (LL671_11210) 2432079..2432327 - 249 WP_005691639.1 hypothetical protein -
LL671_RS11210 (LL671_11215) 2432889..2433161 + 273 WP_014571130.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
LL671_RS11215 (LL671_11220) 2433189..2433374 + 186 WP_005691636.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
LL671_RS11220 (LL671_11225) 2433598..2435013 + 1416 WP_005691634.1 exonuclease domain-containing protein -
LL671_RS11225 (LL671_11230) 2435256..2436131 - 876 WP_024305787.1 XRE family transcriptional regulator -
LL671_RS11230 (LL671_11235) 2436578..2437186 + 609 WP_005691630.1 hypothetical protein -
LL671_RS11235 (LL671_11240) 2437678..2438367 - 690 WP_005691628.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2427363..2438367 11004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-57)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-65)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6781.86 Da        Isoelectric Point: 7.4698

>T222966 WP_005691636.1 NZ_CP086326:2433189-2433374 [Lacticaseibacillus rhamnosus]
MQRQGHVKLASLHDHALKGAHSGERALRVAPDWLLVYKVDAEALILMLLATGTHRDTLNIE

Download         Length: 186 bp

>T222966 NZ_CP118223:c4604397-4604140 [Klebsiella grimontii]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACGTTCGCGATGCATC
AGGCCGCGTCACTTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTATCGCATCAATACCACCGGGCTGAATAATGTCCGCGCA
CAGCTGGATAATCGCTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10005.62 Da        Isoelectric Point: 9.5939

>AT222966 WP_014571130.1 NZ_CP086326:2432889-2433161 [Lacticaseibacillus rhamnosus]
MTKKSRISVRIDTKTKERALHVLNSMGLDISSAINMYLKRIGDTGALPFTPPMSFVDQLQVAEADVKAGRIKSFKTVDAL
MKDLYSDVDD

Download         Length: 273 bp

>AT222966 NZ_CP118223:c4604129-4603578 [Klebsiella grimontii]
ATGACAAACCACAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCAATGACATTCTCCATGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGCGCCTGCCCGACGTTATCGCTGCTGGCCA
GGCATAACGGAAAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTTGCGGTGGTGCCGGAATACCAGGGCGCGGGCGTTGGCGGAGGGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCCATGGGAAGCCAGACGGTATTTGTTCTGGGCCACGCCACTTACTACCCCAGGCATGGGTTTGAGCCATGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCCATTCCCGAGGCGCTCAAAGCCTGCTGGATGCTGCAGCCGCTTTCATCT
CAGCCGCTGGGACGAACGGGTCAAATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X2J6C9

Antitoxin

Source ID Structure
AlphaFold DB A0A508YYT1

References