Detailed information of TA system TA025721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/-
• Location: 131583..132367
• Replicon: chromosome
• Accession: NC_016446
• Organism: Vibrio cholerae O1 str. 2010EL-1786

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: HJ37_RS14900
• Protein ID: WP_001114075.1
• Coordinates: 131583..131852 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: Q9KM84
• Locus tag: HJ37_RS14905
• Protein ID: WP_000921691.1
• Coordinates: 131846..132367 (+)
• Length: 174 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ37_RS14865	126949..127866	+	918	WP_000186333.1	alpha/beta hydrolase	-
HJ37_RS14870	128023..128412	+	390	WP_001081302.1	hypothetical protein	-
HJ37_RS14875	128548..128757	+	210	Protein_202	GNAT family N-acetyltransferase	-
HJ37_RS14880	128876..129313	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
HJ37_RS14885	129377..129595	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
HJ37_RS14890	129770..130642	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
HJ37_RS14895	130792..131391	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
HJ37_RS20045	131448..131552	+	105	WP_099607150.1	acetyltransferase	-
HJ37_RS14900	131583..131852	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
HJ37_RS14905	131846..132367	+	522	WP_000921691.1	DUF2442 domain-containing protein	Antitoxin
HJ37_RS20050	132666..132791	+	126	WP_001905700.1	DUF645 family protein	-
HJ37_RS14910	133042..133437	+	396	WP_001000867.1	hypothetical protein	-
HJ37_RS14915	133576..133854	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
HJ37_RS14920	133851..134135	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
HJ37_RS14925	134329..134844	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
HJ37_RS14930	135028..135441	+	414	WP_000049420.1	VOC family protein	-
HJ37_RS14940	135996..137173	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31115..136304	105189
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	catB9	-	36195..135928	99733
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	128876..129313	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Protein of unknown function DUF2442 (4-59)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T25721 WP_001114075.1 NC_016446:131583-131852 [Vibrio cholerae O1 str. 2010EL-1786]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T25721 NC_016446:131583-131852 [Vibrio cholerae O1 str. 2010EL-1786]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin

Protein

• Length: 174 a.a.
• Molecular weight: 19409.01 Da
• Isoelectric Point: 6.7342

>AT25721 WP_000921691.1 NC_016446:131846-132367 [Vibrio cholerae O1 str. 2010EL-1786]
MLKVIDVDFVSDHTLELTFNDGYQGYADLSVYFKKAPFSEIKDFKRFSLTRDGSLNWDGNELTAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Nucleotide

• Length: 522 bp

>AT25721 NC_016446:131846-132367 [Vibrio cholerae O1 str. 2010EL-1786]
ATGTTGAAAGTCATAGATGTTGATTTTGTCTCAGACCATACATTAGAACTGACGTTCAATGATGGTTACCAAGGCTATGC
CGACTTGTCGGTATACTTTAAAAAAGCACCGTTTTCAGAAATTAAGGACTTTAAACGCTTTTCCTTGACTCGTGATGGCT
CGTTAAATTGGGATGGTAATGAGTTAACGGCCGCGACACTCCGTGACATAACCAAGGGCTCACAAAAGTCGGTGGAGTTA
AGTTTTAATGTTCAAGAAATGGAAGCCGTCATCAAACAGGCCTCATGGGAATCGATGATGGAGGGGCGTCCCGACATCTT
GCAAGCGGCTATTCGTTCTTACGTAGAGCAGTTTGGTCATGGTCAAGTTATTGCAAAGGCGGGTATAAAAAGTCGTACGA
GTGCTTATCGCTCCTTGAAGCCAGAAACAACGCCAAACTTTGGCACACTAGTTCAGTTAGGGCACGCCGTCATCGAACTA
GCGAAAGATAGAACAGTGGCAAACAAGGAACCACGCATATAA

Structures

Toxin

• AlphaFold DB: A0A086SLD6

Antitoxin

• AlphaFold DB: Q9KM84