177

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) /RHH-GNAT
Location 424470..425425 Replicon chromosome II
Accession NC_002506
Organism Vibrio cholerae O1 biovar El Tor str. N16961

Toxin (Protein)


Gene name - Uniprot ID Q9KM94
Locus tag VC_RS15750 Protein ID WP_000118351.1
Coordinates 424892..425425 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID -
Locus tag VC_RS15745 Protein ID WP_001882332.1
Coordinates 424470..424895 (+) Length 142 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VC_RS15700 (VC_A0477) 420000..420251 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VC_RS15705 (VC_A0478) 420241..420528 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
VC_RS15710 (VC_A0479) 420656..421111 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
VC_RS15715 (VC_A0480) 421433..421585 + 153 WP_001884520.1 DUF645 family protein -
VC_RS15720 (VC_A0481) 421787..422284 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
VC_RS15725 (VC_A0482) 422281..422553 - 273 WP_000246253.1 DUF1778 domain-containing protein -
VC_RS15730 (VC_A0483) 422784..423452 + 669 WP_000043871.1 hypothetical protein -
VC_RS15735 (VC_A0484) 423604..423891 + 288 WP_000426470.1 hypothetical protein -
VC_RS15740 (VC_A0485) 424032..424403 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
VC_RS15745 (VC_A0486) 424470..424895 + 426 WP_001882332.1 DUF1778 domain-containing protein Antitoxin
VC_RS15750 (VC_A0487) 424892..425425 + 534 WP_000118351.1 GNAT family N-acetyltransferase Toxin
VC_RS15760 (VC_A0488) 425627..425884 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VC_RS15765 (VC_A0489) 425872..426174 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
VC_RS15770 (VC_A0490) 426408..427325 + 918 WP_000186333.1 alpha/beta hydrolase -
VC_RS15775 (VC_A0491) 427482..427871 + 390 WP_001081302.1 hypothetical protein -
VC_RS15780 (VC_A0492) 428007..428216 + 210 Protein_466 GNAT family N-acetyltransferase -
VC_RS15785 (VC_A0493) 428335..428772 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
VC_RS15790 (VC_A0494) 428836..429054 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
VC_RS15795 (VC_A0495) 429229..430101 + 873 WP_000254716.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 428335..428772 437
inside Integron catB9 - 309750..435387 125637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(97-153)

Antitoxin

Vibrio phage ICP1, Orf50

(57-133)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 20171.46 Da        Isoelectric Point: 9.1432

>T177 WP_000118351.1 NC_002506:424892-425425 [Vibrio cholerae O1 biovar El Tor str. N16961]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Download         Length: 534 bp

>T177 NC_002506:424892-425425 [Vibrio cholerae O1 biovar El Tor str. N16961]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin


Download         Length: 142 a.a.        Molecular weight: 15714.41 Da        Isoelectric Point: 7.6659

>AT177 WP_001882332.1 NC_002506:424470-424895 [Vibrio cholerae O1 biovar El Tor str. N16961]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Download         Length: 426 bp

>AT177 NC_002506:424470-424895 [Vibrio cholerae O1 biovar El Tor str. N16961]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10041 Mycobacterium tuberculosis H37Rv

30.675

98.193

0.301

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9KM94

Antitoxin

Source ID Structure

References

(1) Naeem Iqbal et al. (2015) Comprehensive Functional Analysis of the 18 Vibrio cholerae N16961 Toxin-Antitoxin Systems Substantiates Their Role in Stabilizing the Superintegron. Journal of Bacteriology 197(13):2150-9. [PubMed:25897030] experimental


experimental experimental literature