145915

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /DUF1778(antitoxin)
Location 422119..422918 Replicon chromosome
Accession NZ_CP047306
Organism Vibrio cholerae O37 strain ATCC 25872

Toxin (Protein)


Gene name - Uniprot ID Q9KM94
Locus tag GTH07_RS15610 Protein ID WP_000118351.1
Coordinates 422385..422918 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0K9UER4
Locus tag GTH07_RS18740 Protein ID WP_000179600.1
Coordinates 422119..422388 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH07_RS15560 (GTH07_15635) 417492..417743 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTH07_RS15565 (GTH07_15640) 417733..418020 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTH07_RS15570 (GTH07_15645) 418148..418603 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
GTH07_RS18730 418660..418782 + 123 Protein_464 acetyltransferase -
GTH07_RS15575 (GTH07_15650) 418953..419077 + 125 Protein_465 DUF645 family protein -
GTH07_RS15580 (GTH07_15655) 419280..419777 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
GTH07_RS15585 (GTH07_15660) 419774..420046 - 273 WP_000246253.1 DUF1778 domain-containing protein -
GTH07_RS18735 420198..420260 + 63 Protein_468 acetyltransferase -
GTH07_RS15590 (GTH07_15665) 420277..420945 + 669 WP_000043871.1 hypothetical protein -
GTH07_RS15595 (GTH07_15670) 421097..421384 + 288 WP_000426470.1 hypothetical protein -
GTH07_RS15600 (GTH07_15675) 421525..421896 + 372 WP_001164080.1 MazG nucleotide pyrophosphohydrolase domain-containing protein -
GTH07_RS18740 (GTH07_15680) 422119..422388 + 270 WP_000179600.1 DUF1778 domain-containing protein Antitoxin
GTH07_RS15610 (GTH07_15685) 422385..422918 + 534 WP_000118351.1 GNAT family N-acetyltransferase Toxin
GTH07_RS18745 (GTH07_15690) 422928..423038 + 111 WP_086010065.1 DUF3265 domain-containing protein -
GTH07_RS15615 (GTH07_15695) 423120..423377 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTH07_RS15620 (GTH07_15700) 423365..423667 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTH07_RS15625 (GTH07_15705) 423901..424818 + 918 WP_000186333.1 alpha/beta hydrolase -
GTH07_RS15630 (GTH07_15710) 425026..425268 + 243 WP_000107462.1 hypothetical protein -
GTH07_RS15635 (GTH07_15715) 425443..425832 + 390 WP_001081302.1 hypothetical protein -
GTH07_RS15640 (GTH07_15720) 425968..426177 + 210 Protein_480 GNAT family N-acetyltransferase -
GTH07_RS15645 (GTH07_15725) 426296..426733 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
GTH07_RS15650 (GTH07_15730) 426794..427015 + 222 Protein_482 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 307620..433724 126104
- inside Integron - - 312700..433348 120648
flank IS/Tn - - 426296..426733 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(97-153)

Antitoxin

Vibrio phage ICP1, Orf50

(5-81)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 20171.46 Da        Isoelectric Point: 9.1432

>T145915 WP_000118351.1 NZ_CP047306:422385-422918 [Vibrio cholerae O37]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Download         Length: 534 bp

>T145915 NZ_CP062708:c4713316-4713002 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGTAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9924.49 Da        Isoelectric Point: 4.9107

>AT145915 WP_000179600.1 NZ_CP047306:422119-422388 [Vibrio cholerae O37]
MATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAV
KFTEKSGIK

Download         Length: 270 bp

>AT145915 NZ_CP062708:c4713552-4713319 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9KM94

Antitoxin

Source ID Structure
AlphaFold DB A0A0K9UER4

References