10035

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) DhiAT/-
Location 431042..431826 Replicon chromosome II
Accession NC_002506
Organism Vibrio cholerae O1 biovar El Tor str. N16961

Toxin (Protein)


Gene name dhiT Uniprot ID A0A086SLD6
Locus tag VC_RS15810 Protein ID WP_001114075.1
Coordinates 431042..431311 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name dhiA Uniprot ID Q9KM84
Locus tag VC_RS15815 Protein ID WP_000921691.1
Coordinates 431305..431826 (+) Length 174 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VC_RS15770 (VC_A0490) 426408..427325 + 918 WP_000186333.1 alpha/beta hydrolase -
VC_RS15775 (VC_A0491) 427482..427871 + 390 WP_001081302.1 hypothetical protein -
VC_RS15780 (VC_A0492) 428007..428216 + 210 Protein_466 GNAT family N-acetyltransferase -
VC_RS15785 (VC_A0493) 428335..428772 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
VC_RS15790 (VC_A0494) 428836..429054 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
VC_RS15795 (VC_A0495) 429229..430101 + 873 WP_000254716.1 DUF3800 domain-containing protein -
VC_RS15800 (VC_A0496) 430251..430850 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
VC_RS15805 430907..431011 + 105 WP_099607150.1 acetyltransferase -
VC_RS15810 (VC_A0497) 431042..431311 + 270 WP_001114075.1 DUF4160 domain-containing protein Toxin
VC_RS15815 (VC_A0498) 431305..431826 + 522 WP_000921691.1 DUF2442 domain-containing protein Antitoxin
VC_RS15820 432125..432250 + 126 WP_001905700.1 DUF645 family protein -
VC_RS15825 (VC_A0501) 432501..432896 + 396 WP_001000867.1 hypothetical protein -
VC_RS15830 (VC_A0503) 433035..433313 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
VC_RS15835 (VC_A0504) 433310..433594 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
VC_RS15840 (VC_A0505) 433788..434303 + 516 WP_000343779.1 GNAT family N-acetyltransferase -
VC_RS15845 (VC_A0506) 434487..434900 + 414 WP_000049420.1 VOC family protein -
VC_RS15855 (VC_A0508) 435455..436632 + 1178 WP_086010879.1 IS3-like element ISVch4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 428335..428772 437
inside Integron catB9 - 309750..435387 125637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Protein of unknown function DUF4160

(9-74)

Antitoxin

Protein of unknown function DUF2442

(4-59)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10310.94 Da        Isoelectric Point: 6.6303

>T10035 WP_001114075.1 NC_002506:431042-431311 [Vibrio cholerae O1 biovar El Tor str. N16961]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Download         Length: 270 bp

>T10035 NC_002506:431042-431311 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin


Download         Length: 174 a.a.        Molecular weight: 19409.01 Da        Isoelectric Point: 6.7342

>AT10035 WP_000921691.1 NC_002506:431305-431826 [Vibrio cholerae O1 biovar El Tor str. N16961]
MLKVIDVDFVSDHTLELTFNDGYQGYADLSVYFKKAPFSEIKDFKRFSLTRDGSLNWDGNELTAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Download         Length: 522 bp

>AT10035 NC_002506:431305-431826 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGTTGAAAGTCATAGATGTTGATTTTGTCTCAGACCATACATTAGAACTGACGTTCAATGATGGTTACCAAGGCTATGC
CGACTTGTCGGTATACTTTAAAAAAGCACCGTTTTCAGAAATTAAGGACTTTAAACGCTTTTCCTTGACTCGTGATGGCT
CGTTAAATTGGGATGGTAATGAGTTAACGGCCGCGACACTCCGTGACATAACCAAGGGCTCACAAAAGTCGGTGGAGTTA
AGTTTTAATGTTCAAGAAATGGAAGCCGTCATCAAACAGGCCTCATGGGAATCGATGATGGAGGGGCGTCCCGACATCTT
GCAAGCGGCTATTCGTTCTTACGTAGAGCAGTTTGGTCATGGTCAAGTTATTGCAAAGGCGGGTATAAAAAGTCGTACGA
GTGCTTATCGCTCCTTGAAGCCAGAAACAACGCCAAACTTTGGCACACTAGTTCAGTTAGGGCACGCCGTCATCGAACTA
GCGAAAGATAGAACAGTGGCAAACAAGGAACCACGCATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10066 Dickeya dadantii 3937

60

100

0.6
T10208 Escherichia coli C

37.5

80

0.3

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10066 Dickeya dadantii 3937

37.888

99.383

0.377

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A086SLD6

Antitoxin

Source ID Structure
AlphaFold DB Q9KM84

References

(1) Evelyne Krin et al. (2023) Systematic transcriptome analysis allows the identification of new type I and type II Toxin/Antitoxin systems located in the superintegron of Vibrio cholerae. Research in Microbiology 174(1-2):103997. [PubMed:36347445] experimental


experimental experimental literature