230516

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 324478..325244 Replicon chromosome
Accession NZ_CP090379
Organism Vibrio cholerae strain BY369

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag LVJ30_RS15260 Protein ID WP_000982260.1
Coordinates 324478..324975 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag LVJ30_RS15265 Protein ID WP_000246253.1
Coordinates 324972..325244 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ30_RS15210 (LVJ30_15215) 319920..320624 + 705 WP_000087610.1 HNH endonuclease -
LVJ30_RS15215 (LVJ30_15220) 320782..321120 + 339 WP_000713853.1 hypothetical protein -
LVJ30_RS15220 (LVJ30_15225) 321274..321591 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
LVJ30_RS15225 (LVJ30_15230) 321610..321852 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ30_RS15230 (LVJ30_15235) 322096..322482 + 387 WP_001015867.1 NUDIX hydrolase -
LVJ30_RS15235 (LVJ30_15240) 322539..322652 + 114 WP_001900214.1 hypothetical protein -
LVJ30_RS15240 (LVJ30_15245) 322661..322882 + 222 WP_032467793.1 DUF1289 domain-containing protein -
LVJ30_RS15245 (LVJ30_15250) 323140..323676 + 537 WP_000469482.1 GNAT family protein -
LVJ30_RS15250 (LVJ30_15255) 323867..324328 + 462 WP_001903091.1 lipocalin family protein -
LVJ30_RS15255 (LVJ30_15260) 324393..324461 + 69 Protein_284 acetyltransferase -
LVJ30_RS15260 (LVJ30_15265) 324478..324975 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
LVJ30_RS15265 (LVJ30_15270) 324972..325244 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
LVJ30_RS15270 (LVJ30_15275) 325637..325762 + 126 WP_001900195.1 DUF645 family protein -
LVJ30_RS15275 (LVJ30_15280) 326012..326458 + 447 WP_000006157.1 hypothetical protein -
LVJ30_RS15280 (LVJ30_15285) 326606..326875 - 270 WP_000277238.1 type II toxin-antitoxin system YafQ family toxin -
LVJ30_RS15285 (LVJ30_15290) 326868..327146 - 279 WP_001258569.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
LVJ30_RS15290 (LVJ30_15295) 327197..327314 + 118 Protein_291 DUF3265 domain-containing protein -
LVJ30_RS15295 (LVJ30_15300) 327432..327710 + 279 WP_001921601.1 DUF3709 domain-containing protein -
LVJ30_RS15300 (LVJ30_15305) 328070..328168 + 99 WP_223225486.1 DUF3709 domain-containing protein -
LVJ30_RS15305 (LVJ30_15310) 328368..328469 + 102 WP_001921603.1 hypothetical protein -
LVJ30_RS15310 (LVJ30_15315) 328459..328845 + 387 WP_000703163.1 VOC family protein -
LVJ30_RS15315 (LVJ30_15320) 329040..329291 + 252 WP_001890111.1 TIGR03643 family protein -
LVJ30_RS15320 (LVJ30_15325) 329264..329413 + 150 WP_071908333.1 DUF3265 domain-containing protein -
LVJ30_RS15325 (LVJ30_15330) 329505..329747 + 243 WP_000107461.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304645..399892 95247
inside Integron - - 309725..399516 89791


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T230516 WP_000982260.1 NZ_CP090379:c324975-324478 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T230516 NZ_CP124916:411859-412167 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT230516 WP_000246253.1 NZ_CP090379:c325244-324972 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT230516 NZ_CP124916:411585-411857 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References