10030

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) phd-doc/-
Location 418092..419185 Replicon chromosome II
Accession NC_002506
Organism Vibrio cholerae O1 biovar El Tor str. N16961

Toxin (Protein)


Gene name doc Uniprot ID Q9KMA2
Locus tag VC_RS15685 Protein ID WP_000351248.1
Coordinates 418784..419185 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID -
Locus tag VC_RS15680 Protein ID WP_001047169.1
Coordinates 418092..418784 (+) Length 231 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VC_RS15620 (VC_A0465) 413375..413752 + 378 WP_000411109.1 hypothetical protein -
VC_RS18765 413809..413923 + 115 Protein_437 acetyltransferase -
VC_RS15625 413872..414153 + 282 WP_001894453.1 DUF1289 domain-containing protein -
VC_RS15635 414319..414432 + 114 WP_001889158.1 hypothetical protein -
VC_RS15640 414381..414608 + 228 WP_001894457.1 DUF1289 domain-containing protein -
VC_RS15650 (VC_A0468) 414949..415281 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin -
VC_RS15655 (VC_A0469) 415268..415582 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
VC_RS15660 (VC_A0470) 415719..416153 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
VC_RS15665 (VC_A0471) 416321..416476 + 156 WP_000751734.1 hypothetical protein -
VC_RS15670 (VC_A0472) 416601..417581 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
VC_RS15675 (VC_A0473) 417649..417955 + 307 Protein_446 CatB-related O-acetyltransferase -
VC_RS15680 (VC_A0474) 418092..418784 + 693 WP_001047169.1 GNAT family N-acetyltransferase Antitoxin
VC_RS15685 (VC_A0475) 418784..419185 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
VC_RS15690 419155..419298 + 144 WP_071908341.1 DUF3265 domain-containing protein -
VC_RS15695 (VC_A0476) 419373..419786 + 414 WP_000049417.1 VOC family protein -
VC_RS15700 (VC_A0477) 420000..420251 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VC_RS15705 (VC_A0478) 420241..420528 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
VC_RS15710 (VC_A0479) 420656..421111 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
VC_RS15715 (VC_A0480) 421433..421585 + 153 WP_001884520.1 DUF645 family protein -
VC_RS15720 (VC_A0481) 421787..422284 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
VC_RS15725 (VC_A0482) 422281..422553 - 273 WP_000246253.1 DUF1778 domain-containing protein -
VC_RS15730 (VC_A0483) 422784..423452 + 669 WP_000043871.1 hypothetical protein -
VC_RS15735 (VC_A0484) 423604..423891 + 288 WP_000426470.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 416601..417581 980
inside Integron catB9 - 309750..435387 125637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-132)

Antitoxin

GNAT domain

(36-153)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14608.71 Da        Isoelectric Point: 4.0128

>T10030 WP_000351248.1 NC_002506:418784-419185 [Vibrio cholerae O1 biovar El Tor str. N16961]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Download         Length: 402 bp

>T10030 NC_002506:418784-419185 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin


Download         Length: 231 a.a.        Molecular weight: 26352.73 Da        Isoelectric Point: 8.5633

>AT10030 WP_001047169.1 NC_002506:418092-418784 [Vibrio cholerae O1 biovar El Tor str. N16961]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Download         Length: 693 bp

>AT10030 NC_002506:418092-418784 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0PN34

Antitoxin

Source ID Structure

References

(1) Anne-Marie Guérout et al. (2013) Characterization of the phd-doc and ccd toxin-antitoxin cassettes from Vibrio superintegrons. Journal of Bacteriology 195(10):2270-83. [PubMed:23475970] experimental


experimental experimental literature