Detailed information of TA system TA056925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 397164..397723
• Replicon: chromosome
• Accession: NZ_CP012666
• Organism: Vibrio cholerae strain ICDC-VC661

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q9K2M8
• Locus tag: AN947_RS02340
• Protein ID: WP_000578476.1
• Coordinates: 397164..397442 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9L991
• Locus tag: AN947_RS02345
• Protein ID: WP_000381183.1
• Coordinates: 397439..397723 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN947_RS02290	392464..392901	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
AN947_RS02295	392965..393183	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
AN947_RS02300	393358..394230	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
AN947_RS02305	394380..394979	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
AN947_RS20790	395036..395140	+	105	WP_099607150.1	acetyltransferase	-
AN947_RS02310	395171..395440	+	270	WP_001114075.1	DUF4160 domain-containing protein	-
AN947_RS02315	395434..395955	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
AN947_RS02325	396254..396379	+	126	WP_001905700.1	DUF645 family protein	-
AN947_RS02335	396630..397025	+	396	WP_001000867.1	hypothetical protein	-
AN947_RS02340	397164..397442	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
AN947_RS02345	397439..397723	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AN947_RS02350	397917..398432	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
AN947_RS02355	398616..399029	+	414	WP_000049420.1	VOC family protein	-
AN947_RS02365	399584..400761	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304645..399892	95247
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309725..399516	89791
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	392464..392901	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10819.59 Da
• Isoelectric Point: 5.1651

>T56925 WP_000578476.1 NZ_CP012666:c397442-397164 [Vibrio cholerae]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Nucleotide

• Length: 279 bp

>T56925 NZ_CP012666:c397442-397164 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10901.25 Da
• Isoelectric Point: 8.0775

>AT56925 WP_000381183.1 NZ_CP012666:c397723-397439 [Vibrio cholerae]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Nucleotide

• Length: 285 bp

>AT56925 NZ_CP012666:c397723-397439 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: Q9K2M8

Antitoxin

• AlphaFold DB: A0A067BL33