Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Xre-RelE
Location 414949..415582 Replicon chromosome II
Accession NC_002506
Organism Vibrio cholerae O1 biovar El Tor str. N16961

Toxin (Protein)


Gene name higB Uniprot ID Q9KMA6
Locus tag VC_RS15650 Protein ID WP_000843587.1
Coordinates 414949..415281 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag VC_RS15655 Protein ID WP_000071008.1
Coordinates 415268..415582 (+) Length 105 a.a.

Genomic Context

VC_RS15590VC_RS15595VC_RS15600VC_RS18760VC_RS15610VC_RS15615VC_RS15620VC_RS18765VC_RS15625VC_RS15635VC_RS15640VC_RS15650VC_RS15655VC_RS15660VC_RS15665VC_RS15675VC_RS15680VC_RS15685VC_RS15690VC_RS15695VC_RS15700VC_RS15705VC_RS15670
Location: 410390..410593 (204 bp)
Type: Others
Protein ID: WP_001911745.1
Location: 410874..411047 (174 bp)
Type: Others
Protein ID: WP_001882305.1
Location: 411407..411676 (270 bp)
Type: Others
Protein ID: WP_001198131.1
Location: 411994..412165 (172 bp)
Type: Others
Protein ID: Protein_433
Location: 412374..412760 (387 bp)
Type: Others
Protein ID: WP_000703163.1
Location: 412962..413204 (243 bp)
Type: Others
Protein ID: WP_000107461.1
Location: 413375..413752 (378 bp)
Type: Others
Protein ID: WP_000411109.1
Location: 413809..413923 (115 bp)
Type: Others
Protein ID: Protein_437
Location: 413872..414153 (282 bp)
Type: Others
Protein ID: WP_001894453.1
Location: 414319..414432 (114 bp)
Type: Others
Protein ID: WP_001889158.1
Location: 414381..414608 (228 bp)
Type: Others
Protein ID: WP_001894457.1
Location: 414949..415281 (333 bp)
Type: Toxin
Protein ID: WP_000843587.1
Location: 415268..415582 (315 bp)
Type: Antitoxin
Protein ID: WP_000071008.1
Location: 415719..416153 (435 bp)
Type: Others
Protein ID: WP_000256036.1
Location: 416321..416476 (156 bp)
Type: Others
Protein ID: WP_000751734.1
Location: 417649..417955 (307 bp)
Type: Others
Protein ID: Protein_446
Location: 418092..418784 (693 bp)
Type: Others
Protein ID: WP_001047169.1
Location: 418784..419185 (402 bp)
Type: Others
Protein ID: WP_000351248.1
Location: 419155..419298 (144 bp)
Type: Others
Protein ID: WP_071908341.1
Location: 419373..419786 (414 bp)
Type: Others
Protein ID: WP_000049417.1
Location: 420000..420251 (252 bp)
Type: Others
Protein ID: WP_001250179.1
Location: 420241..420528 (288 bp)
Type: Others
Protein ID: WP_000221354.1
Location: 416601..417581 (981 bp)
Type: Others
Protein ID: WP_000019370.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
VC_RS15590410390..410593+204WP_001911745.1hypothetical protein-
VC_RS15595410874..411047+174WP_001882305.1DUF3709 domain-containing protein-
VC_RS15600411407..411676+270WP_001198131.1hypothetical protein-
VC_RS18760411994..412165+172Protein_433DUF645 family protein-
VC_RS15610 (VC_A0463)412374..412760+387WP_000703163.1VOC family protein-
VC_RS15615412962..413204+243WP_000107461.1hypothetical protein-
VC_RS15620 (VC_A0465)413375..413752+378WP_000411109.1hypothetical protein-
VC_RS18765413809..413923+115Protein_437acetyltransferase-
VC_RS15625413872..414153+282WP_001894453.1DUF1289 domain-containing protein-
VC_RS15635414319..414432+114WP_001889158.1hypothetical protein-
VC_RS15640414381..414608+228WP_001894457.1DUF1289 domain-containing protein-
VC_RS15650 (VC_A0468)414949..415281+333WP_000843587.1type II toxin-antitoxin system RelE/ParE family toxinToxin
VC_RS15655 (VC_A0469)415268..415582+315WP_000071008.1DNA-binding transcriptional regulatorAntitoxin
VC_RS15660 (VC_A0470)415719..416153+435WP_000256036.1GNAT family N-acetyltransferase-
VC_RS15665 (VC_A0471)416321..416476+156WP_000751734.1hypothetical protein-
VC_RS15670 (VC_A0472)416601..417581-981WP_000019370.1IS5-like element ISVch5 family transposase-
VC_RS15675 (VC_A0473)417649..417955+307Protein_446CatB-related O-acetyltransferase-
VC_RS15680 (VC_A0474)418092..418784+693WP_001047169.1GNAT family N-acetyltransferase-
VC_RS15685 (VC_A0475)418784..419185+402WP_000351248.1type II toxin-antitoxin system death-on-curing family toxin-
VC_RS15690419155..419298+144WP_071908341.1DUF3265 domain-containing protein-
VC_RS15695 (VC_A0476)419373..419786+414WP_000049417.1VOC family protein-
VC_RS15700 (VC_A0477)420000..420251+252WP_001250179.1type II toxin-antitoxin system Phd/YefM family antitoxin-
VC_RS15705 (VC_A0478)420241..420528+288WP_000221354.1type II toxin-antitoxin system RelE/ParE family toxin-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
flankIS/Tn--416601..417581980
insideIntegroncatB9-309750..435387125637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(4-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(43-92)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 13005.95 Da        Isoelectric Point: 9.9244

>T174 WP_000843587.1 NC_002506:414949-415281 [Vibrio cholerae O1 biovar El Tor str. N16961]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Download         Length: 333 bp

>T174 NC_002506:414949-415281 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11696.20 Da        Isoelectric Point: 6.7600

>AT174 WP_000071008.1 NC_002506:415268-415582 [Vibrio cholerae O1 biovar El Tor str. N16961]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Download         Length: 315 bp

>AT174 NC_002506:415268-415582 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
T303Pseudomonas putida KT2440

42.718

99.038

0.423

T6088Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

40.594

98.058

0.398
Showing 1 to 2 of 2 rows

Multiple sequence alignment    


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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found

Structures

Toxin

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Source
ID
Structure
PDB5JA9
PDB8A0X
PDB5MJE
PDB5JA8
PDB5JAA

Antitoxin

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Source
ID
Structure
No matching records found

References

(1) Evelyne Krin et al. (2023) Systematic transcriptome analysis allows the identification of new type I and type II Toxin/Antitoxin systems located in the superintegron of Vibrio cholerae. Research in Microbiology 174(1-2):103997. [PubMed:36347445] experimental

(2) San Hadži et al. (2017) Ribosome-dependent Vibrio cholerae mRNAse HigB2 is regulated by a β-strand sliding mechanism. Nucleic Acids Research 45(8):4972-4983. [PubMed:28334932] 3D_structure

(3) Naeem Iqbal et al. (2015) Comprehensive Functional Analysis of the 18 Vibrio cholerae N16961 Toxin-Antitoxin Systems Substantiates Their Role in Stabilizing the Superintegron. Journal of Bacteriology 197(13):2150-9. [PubMed:25897030] experimental

(4) Uroš Zavrtanik et al. (2021) Unraveling the Thermodynamics of Ultra-tight Binding of Intrinsically Disordered Proteins. Frontiers in Molecular Biosciences 8:726824. [PubMed:34532345] experimental


experimental experimental literature
3D_structure protein structure literature