174

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Xre-RelE
Location 414949..415582 Replicon chromosome II
Accession NC_002506
Organism Vibrio cholerae O1 biovar El Tor str. N16961

Toxin (Protein)


Gene name higB Uniprot ID Q9KMA6
Locus tag VC_RS15650 Protein ID WP_000843587.1
Coordinates 414949..415281 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag VC_RS15655 Protein ID WP_000071008.1
Coordinates 415268..415582 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VC_RS15590 410390..410593 + 204 WP_001911745.1 hypothetical protein -
VC_RS15595 410874..411047 + 174 WP_001882305.1 DUF3709 domain-containing protein -
VC_RS15600 411407..411676 + 270 WP_001198131.1 hypothetical protein -
VC_RS18760 411994..412165 + 172 Protein_433 DUF645 family protein -
VC_RS15610 (VC_A0463) 412374..412760 + 387 WP_000703163.1 VOC family protein -
VC_RS15615 412962..413204 + 243 WP_000107461.1 hypothetical protein -
VC_RS15620 (VC_A0465) 413375..413752 + 378 WP_000411109.1 hypothetical protein -
VC_RS18765 413809..413923 + 115 Protein_437 acetyltransferase -
VC_RS15625 413872..414153 + 282 WP_001894453.1 DUF1289 domain-containing protein -
VC_RS15635 414319..414432 + 114 WP_001889158.1 hypothetical protein -
VC_RS15640 414381..414608 + 228 WP_001894457.1 DUF1289 domain-containing protein -
VC_RS15650 (VC_A0468) 414949..415281 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
VC_RS15655 (VC_A0469) 415268..415582 + 315 WP_000071008.1 DNA-binding transcriptional regulator Antitoxin
VC_RS15660 (VC_A0470) 415719..416153 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
VC_RS15665 (VC_A0471) 416321..416476 + 156 WP_000751734.1 hypothetical protein -
VC_RS15670 (VC_A0472) 416601..417581 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
VC_RS15675 (VC_A0473) 417649..417955 + 307 Protein_446 CatB-related O-acetyltransferase -
VC_RS15680 (VC_A0474) 418092..418784 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
VC_RS15685 (VC_A0475) 418784..419185 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
VC_RS15690 419155..419298 + 144 WP_071908341.1 DUF3265 domain-containing protein -
VC_RS15695 (VC_A0476) 419373..419786 + 414 WP_000049417.1 VOC family protein -
VC_RS15700 (VC_A0477) 420000..420251 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VC_RS15705 (VC_A0478) 420241..420528 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 416601..417581 980
inside Integron catB9 - 309750..435387 125637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(4-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(43-92)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 13005.95 Da        Isoelectric Point: 9.9244

>T174 WP_000843587.1 NC_002506:414949-415281 [Vibrio cholerae O1 biovar El Tor str. N16961]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Download         Length: 333 bp

>T174 NC_002506:414949-415281 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11696.20 Da        Isoelectric Point: 6.7600

>AT174 WP_000071008.1 NC_002506:415268-415582 [Vibrio cholerae O1 biovar El Tor str. N16961]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Download         Length: 315 bp

>AT174 NC_002506:415268-415582 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T303 Pseudomonas putida KT2440

42.718

99.038

0.423
T6088 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

40.594

98.058

0.398

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5JA9
PDB 8A0X
PDB 5MJE
PDB 5JA8
PDB 5JAA

Antitoxin

Source ID Structure

References

(1) Evelyne Krin et al. (2023) Systematic transcriptome analysis allows the identification of new type I and type II Toxin/Antitoxin systems located in the superintegron of Vibrio cholerae. Research in Microbiology 174(1-2):103997. [PubMed:36347445] experimental

(2) San Hadži et al. (2017) Ribosome-dependent Vibrio cholerae mRNAse HigB2 is regulated by a β-strand sliding mechanism. Nucleic Acids Research 45(8):4972-4983. [PubMed:28334932] 3D_structure

(3) Naeem Iqbal et al. (2015) Comprehensive Functional Analysis of the 18 Vibrio cholerae N16961 Toxin-Antitoxin Systems Substantiates Their Role in Stabilizing the Superintegron. Journal of Bacteriology 197(13):2150-9. [PubMed:25897030] experimental

(4) Uroš Zavrtanik et al. (2021) Unraveling the Thermodynamics of Ultra-tight Binding of Intrinsically Disordered Proteins. Frontiers in Molecular Biosciences 8:726824. [PubMed:34532345] experimental


experimental experimental literature
3D_structure protein structure literature