Detailed information of TA system TA135200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 848282..849375
• Replicon: chromosome
• Accession: NZ_CP042300
• Organism: Vibrio cholerae O1 strain AAS91

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: FKV26_RS17240
• Protein ID: WP_000351248.1
• Coordinates: 848282..848683 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: FKV26_RS17245
• Protein ID: WP_001047169.1
• Coordinates: 848683..849375 (-)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FKV26_RS17190	843306..844286	-	981	WP_000019368.1	IS5-like element ISVch5 family transposase	-
FKV26_RS17195	844356..844682	-	327	Protein_745	hypothetical protein	-
FKV26_RS17200	844913..845185	+	273	WP_000246253.1	DUF1778 domain-containing protein	-
FKV26_RS17205	845182..845679	+	498	WP_000982260.1	GNAT family N-acetyltransferase	-
FKV26_RS17210	845882..846034	-	153	WP_001884520.1	DUF645 family protein	-
FKV26_RS17215	846356..846811	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
FKV26_RS17220	846939..847226	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FKV26_RS17225	847216..847467	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FKV26_RS17230	847681..848094	-	414	WP_000049417.1	VOC family protein	-
FKV26_RS17235	848169..848312	-	144	WP_071908341.1	DUF3265 domain-containing protein	-
FKV26_RS17240	848282..848683	-	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
FKV26_RS17245	848683..849375	-	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
FKV26_RS17250	849512..849818	-	307	Protein_756	CatB-related O-acetyltransferase	-
FKV26_RS17255	849886..850866	+	981	WP_000019368.1	IS5-like element ISVch5 family transposase	-
FKV26_RS18965	850991..851191	-	201	WP_001198130.1	hypothetical protein	-
FKV26_RS17260	851551..851724	-	174	WP_001882305.1	DUF3709 domain-containing protein	-
FKV26_RS17265	852017..852568	-	552	WP_000821930.1	hypothetical protein	-
FKV26_RS17270	852724..853581	-	858	WP_001279526.1	DUF2806 domain-containing protein	-
FKV26_RS17275	853728..854342	-	615	WP_000789577.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	827249..947639	120390
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0.001	flank	IS/Tn	-	-	843306..844286	980
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0.001	flank	IS/Tn	-	-	849886..850866	980
	-	inside	Integron	-	-	828494..941258	112764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T135200 WP_000351248.1 NZ_CP042300:c848683-848282 [Vibrio cholerae O1]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T135200 NZ_CP042300:c848683-848282 [Vibrio cholerae O1]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT135200 WP_001047169.1 NZ_CP042300:c849375-848683 [Vibrio cholerae O1]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT135200 NZ_CP042300:c849375-848683 [Vibrio cholerae O1]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34