145912

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc(toxin)
Location 416107..416678 Replicon chromosome
Accession NZ_CP047306
Organism Vibrio cholerae O37 strain ATCC 25872

Toxin (Protein)


Gene name doc Uniprot ID A0A0F2H0W6
Locus tag GTH07_RS15550 Protein ID WP_000351251.1
Coordinates 416277..416678 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID Q8GNG3
Locus tag GTH07_RS18720 Protein ID WP_001080654.1
Coordinates 416107..416277 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH07_RS15495 411302..411416 + 115 Protein_446 acetyltransferase -
GTH07_RS15500 (GTH07_15565) 411425..411646 + 222 WP_032468461.1 DUF1289 domain-containing protein -
GTH07_RS15505 (GTH07_15575) 411812..411925 + 114 WP_001889158.1 hypothetical protein -
GTH07_RS15510 (GTH07_15580) 411874..412101 + 228 WP_001894457.1 DUF1289 domain-containing protein -
GTH07_RS15515 (GTH07_15590) 412442..412774 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTH07_RS15520 (GTH07_15595) 412761..413075 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
GTH07_RS15525 (GTH07_15600) 413212..413646 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
GTH07_RS15530 (GTH07_15605) 413814..413969 + 156 WP_000751734.1 hypothetical protein -
GTH07_RS15535 (GTH07_15610) 414094..415074 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
GTH07_RS15540 (GTH07_15615) 415142..415448 + 307 Protein_455 CatB-related O-acetyltransferase -
GTH07_RS18715 415585..415983 + 399 Protein_456 GNAT family N-acetyltransferase -
GTH07_RS18720 416107..416277 + 171 WP_001080654.1 hypothetical protein Antitoxin
GTH07_RS15550 (GTH07_15625) 416277..416678 + 402 WP_000351251.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
GTH07_RS18725 416648..416790 + 143 Protein_459 DUF3265 domain-containing protein -
GTH07_RS15555 (GTH07_15630) 416865..417278 + 414 WP_000049417.1 VOC family protein -
GTH07_RS15560 (GTH07_15635) 417492..417743 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTH07_RS15565 (GTH07_15640) 417733..418020 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTH07_RS15570 (GTH07_15645) 418148..418603 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
GTH07_RS18730 418660..418782 + 123 Protein_464 acetyltransferase -
GTH07_RS15575 (GTH07_15650) 418953..419077 + 125 Protein_465 DUF645 family protein -
GTH07_RS15580 (GTH07_15655) 419280..419777 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
GTH07_RS15585 (GTH07_15660) 419774..420046 - 273 WP_000246253.1 DUF1778 domain-containing protein -
GTH07_RS18735 420198..420260 + 63 Protein_468 acetyltransferase -
GTH07_RS15590 (GTH07_15665) 420277..420945 + 669 WP_000043871.1 hypothetical protein -
GTH07_RS15595 (GTH07_15670) 421097..421384 + 288 WP_000426470.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 307620..433724 126104
- inside Integron - - 312700..433348 120648
flank IS/Tn - - 414094..415074 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(10-106)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14574.70 Da        Isoelectric Point: 4.0128

>T145912 WP_000351251.1 NZ_CP047306:416277-416678 [Vibrio cholerae O37]
MDIICFPFERVIEINALILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Download         Length: 402 bp

>T145912 NZ_CP062708:4218091-4218309 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6287.35 Da        Isoelectric Point: 10.7274

>AT145912 WP_001080654.1 NZ_CP047306:416107-416277 [Vibrio cholerae O37]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Download         Length: 171 bp

>AT145912 NZ_CP062708:4217691-4218065 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2H0W6

Antitoxin

Source ID Structure
AlphaFold DB A0A1T4SJJ1

References