145856

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 425316..426082 Replicon chromosome
Accession NZ_CP047300
Organism Vibrio cholerae O1 biovar El Tor strain P27459

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag GTF71_RS15770 Protein ID WP_000982260.1
Coordinates 425316..425813 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag GTF71_RS15775 Protein ID WP_000246253.1
Coordinates 425810..426082 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF71_RS15725 421178..421450 + 273 Protein_455 CatB-related O-acetyltransferase -
GTF71_RS15730 421621..422313 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
GTF71_RS15735 422313..422714 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
GTF71_RS15740 422684..422827 + 144 WP_071908341.1 DUF3265 domain-containing protein -
GTF71_RS15745 422902..423315 + 414 WP_000049417.1 VOC family protein -
GTF71_RS15750 423529..423780 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF71_RS15755 423770..424057 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF71_RS15760 424185..424640 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
GTF71_RS15765 424962..425114 + 153 WP_001884520.1 DUF645 family protein -
GTF71_RS15770 425316..425813 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
GTF71_RS15775 425810..426082 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
GTF71_RS15780 426313..426981 + 669 WP_000043871.1 hypothetical protein -
GTF71_RS15785 427133..427420 + 288 WP_000426470.1 hypothetical protein -
GTF71_RS15790 427561..427932 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
GTF71_RS15795 427999..428424 + 426 WP_001882332.1 DUF1778 domain-containing protein -
GTF71_RS15800 428421..428954 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
GTF71_RS15805 429156..429413 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF71_RS15810 429401..429703 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF71_RS15815 429937..430854 + 918 WP_000186333.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 306926..439292 132366
inside Integron - - 312006..438916 126910
flank IS/Tn - - 420130..421110 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T145856 WP_000982260.1 NZ_CP047300:c425813-425316 [Vibrio cholerae O1 biovar El Tor]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T145856 NZ_CP062702:c4896361-4896011 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT145856 WP_000246253.1 NZ_CP047300:c426082-425810 [Vibrio cholerae O1 biovar El Tor]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT145856 NZ_CP062702:c4896606-4896355 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References