Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 5079..5845 Replicon chromosome
Accession NZ_CP077061
Organism Vibrio cholerae strain MS84A

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag J3992_RS13560 Protein ID WP_000982260.1
Coordinates 5348..5845 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag J3992_RS13555 Protein ID WP_000246253.1
Coordinates 5079..5351 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3992_RS13505 (J3992_13500) 307..1224 - 918 WP_000186333.1 alpha/beta hydrolase -
J3992_RS13510 (J3992_13505) 1458..1760 - 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
J3992_RS13515 (J3992_13510) 1748..2005 - 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
J3992_RS13520 (J3992_13515) 2087..2197 - 111 WP_086010065.1 DUF3265 domain-containing protein -
J3992_RS13525 (J3992_13520) 2207..2740 - 534 WP_000118351.1 GNAT family N-acetyltransferase -
J3992_RS13530 (J3992_13525) 2737..3006 - 270 WP_000179600.1 DUF1778 domain-containing protein -
J3992_RS13535 (J3992_13530) 3229..3600 - 372 WP_001164080.1 MazG nucleotide pyrophosphohydrolase domain-containing protein -
J3992_RS13540 (J3992_13535) 3741..4028 - 288 WP_000426470.1 hypothetical protein -
J3992_RS13545 (J3992_13540) 4180..4848 - 669 WP_000043871.1 hypothetical protein -
J3992_RS13550 (J3992_13545) 4865..4927 - 63 Protein_10 acetyltransferase -
J3992_RS13555 (J3992_13550) 5079..5351 + 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
J3992_RS13560 (J3992_13555) 5348..5845 + 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
J3992_RS13565 (J3992_13560) 5862..5930 - 69 Protein_13 acetyltransferase -
J3992_RS13570 6047..6151 - 105 WP_228840819.1 DUF645 family protein -
J3992_RS13575 (J3992_13570) 6342..6464 - 123 Protein_15 acetyltransferase -
J3992_RS13580 (J3992_13575) 6521..6976 - 456 WP_001245327.1 GNAT family N-acetyltransferase -
J3992_RS13585 (J3992_13580) 7104..7391 - 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
J3992_RS13590 (J3992_13585) 7381..7632 - 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
J3992_RS13595 (J3992_13590) 7846..8259 - 414 WP_000049417.1 VOC family protein -
J3992_RS13600 (J3992_13595) 8334..8444 - 111 WP_082798255.1 DUF3265 domain-containing protein -
J3992_RS13605 (J3992_13600) 8447..8848 - 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
J3992_RS13610 (J3992_13605) 8848..9018 - 171 WP_001080654.1 hypothetical protein -
J3992_RS13615 (J3992_13610) 9142..9540 - 399 Protein_23 GNAT family N-acetyltransferase -
J3992_RS13620 (J3992_13615) 9677..9983 - 307 Protein_24 CatB-related O-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - hlyA / vgrG-3 / vasL / icmF/vasK / vasJ / vasI / vasH / clpB/vasG / vasF / vasE / vasD / vasC / vasB / vasA / VCA0109 / vipB/mglB / vipA/mglA / vgrG-2 / hcp-2 / hlyA / htpB / ugd / cqsA 1..1044173 1044172
flank IS/Tn - - 10051..11031 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T206601 WP_000982260.1 NZ_CP077061:5348-5845 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T206601 NZ_CP102500:c4372559-4372239 [Citrobacter youngae]
ATGCAAATCTCAACTGTACCGGCCACGATGCCGGTTTCGTCACGCCTGTCGCCCGTGCAGGTCTGGCAGCAATTGTTAAC
GTATCTGCTGGAGCACCATTACGGCCTGACGCTCAACGACACACCATTGCATGATGATGCGGCCATCAAGGAACATATTG
AGGCGGGTATCACGCTTGCTGATGCGGTGAATTTCCTGGTGGAACGTTATGAACTGGTACGCACTGATCGCAAGGGGTTT
ACGTGGCAGGAGCAGACACCATTTGTAACCGCTATAGATATTCTCAGAGCCAGAAGAGCTACCGGGCTGATGAATAAGTA
A

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT206601 WP_000246253.1 NZ_CP077061:5079-5351 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT206601 NZ_CP102500:c4372962-4372627 [Citrobacter youngae]
ATGTATAAAGACGCTACACCAACCTGGGGGCTTAGACGAGATATCACTCCGTGTTTCGGAGCCCGACTGGTGCAGGAAGG
TCATCGCCTGCATTTTCTGGCTAACCGGGCAGGATTCACCGGCTCATTCAGTGAGGTGCAGACCTTACAACTGAATGAAG
CCTTCCCGCATTTTGTCGCATATCTGGAGCTGATGCTGCTCTCCTGCGAGCTGAATCCCCGGTACGCGCACTGTGTCACA
CTGTACCGTAACGGGTTGACTTGTGAAGCAGATACGCTGGGCAGTCATGGGCACGTGTATATGGCAATTTATCCCTCAAA
TCAGGTTAAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
PDB 1Y9B
AlphaFold DB A0A0F2IC79

References