Detailed information of TA system TA092512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 415657..416750
• Replicon: chromosome
• Accession: NZ_CP025937
• Organism: Vibrio cholerae strain A1552

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: C1H56_RS16730
• Protein ID: WP_000351248.1
• Coordinates: 416349..416750 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: C1H56_RS16725
• Protein ID: WP_001047169.1
• Coordinates: 415657..416349 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1H56_RS16660	410940..411317	+	378	WP_000411109.1	hypothetical protein	-
C1H56_RS20005	411374..411488	+	115	Protein_435	acetyltransferase	-
C1H56_RS16665	411437..411718	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
C1H56_RS16680	411884..411997	+	114	WP_001889158.1	hypothetical protein	-
C1H56_RS16685	411946..412173	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
C1H56_RS16700	412514..412846	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C1H56_RS16705	412833..413147	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
C1H56_RS16710	413284..413718	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
C1H56_RS19860	413886..414041	+	156	WP_000751734.1	hypothetical protein	-
C1H56_RS16715	414166..415146	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
C1H56_RS16720	415214..415486	+	273	Protein_444	CatB-related O-acetyltransferase	-
C1H56_RS16725	415657..416349	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
C1H56_RS16730	416349..416750	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C1H56_RS16735	416720..416863	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
C1H56_RS16740	416938..417351	+	414	WP_000049417.1	VOC family protein	-
C1H56_RS16745	417565..417816	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C1H56_RS16750	417806..418093	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C1H56_RS16755	418221..418676	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
C1H56_RS16760	418998..419150	+	153	WP_001884520.1	DUF645 family protein	-
C1H56_RS16770	419352..419849	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
C1H56_RS16775	419846..420118	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
C1H56_RS16780	420349..421017	+	669	WP_000043871.1	hypothetical protein	-
C1H56_RS16785	421169..421456	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304067..433328	129261
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	catB9	-	309147..432952	123805
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0.001	flank	IS/Tn	-	-	414166..415146	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T92512 WP_000351248.1 NZ_CP025937:416349-416750 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T92512 NZ_CP025937:416349-416750 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT92512 WP_001047169.1 NZ_CP025937:415657-416349 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT92512 NZ_CP025937:415657-416349 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34