Detailed information of TA system TA023733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 427343..428436
• Replicon: chromosome II
• Accession: NC_012583
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: VC395_RS16450
• Protein ID: WP_000351248.1
• Coordinates: 428035..428436 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: VC395_RS16445
• Protein ID: WP_001047169.1
• Coordinates: 427343..428035 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC395_RS16400	422694..422963	+	270	WP_001198131.1	hypothetical protein	-
VC395_RS21115	423281..423452	+	172	Protein_431	DUF645 family protein	-
VC395_RS20375	423569..423683	+	115	Protein_432	acetyltransferase	-
VC395_RS16405	423632..423859	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
VC395_RS16415	424200..424532	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VC395_RS16420	424519..424833	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
VC395_RS16425	424970..425404	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
VC395_RS21070	425572..425727	+	156	WP_000751734.1	hypothetical protein	-
VC395_RS16435	425852..426832	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
VC395_RS16440	426900..427172	+	273	Protein_439	CatB-related O-acetyltransferase	-
VC395_RS16445	427343..428035	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
VC395_RS16450	428035..428436	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
VC395_RS20655	428406..428549	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
VC395_RS16455	428624..429037	+	414	WP_000049417.1	VOC family protein	-
VC395_RS16460	429251..429502	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VC395_RS16465	429492..429779	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VC395_RS16470	429907..430362	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
VC395_RS16475	430684..430836	+	153	WP_001884520.1	DUF645 family protein	-
VC395_RS16480	431092..431337	-	246	WP_001912198.1	hypothetical protein	-
VC395_RS16485	431550..433298	-	1749	WP_000063386.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	344619..430484	85865
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	425852..426832	980
	-	inside	Prophage	-	-	427343..471178	43835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T23733 WP_000351248.1 NC_012583:428035-428436 [Vibrio cholerae O395]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T23733 NC_012583:428035-428436 [Vibrio cholerae O395]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT23733 WP_001047169.1 NC_012583:427343-428035 [Vibrio cholerae O395]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT23733 NC_012583:427343-428035 [Vibrio cholerae O395]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34