Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 141998..142526 Replicon chromosome
Accession NZ_CP033513
Organism Vibrio cholerae strain E4

Toxin (Protein)


Gene name relE Uniprot ID A0A0X1KZS6
Locus tag EEL44_RS00715 Protein ID WP_000221354.1
Coordinates 141998..142285 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X1KZS8
Locus tag EEL44_RS00720 Protein ID WP_001250179.1
Coordinates 142275..142526 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL44_RS00665 137101..137634 - 534 WP_000118351.1 GNAT family N-acetyltransferase -
EEL44_RS00670 137631..138056 - 426 WP_001882332.1 DUF1778 domain-containing protein -
EEL44_RS00675 138123..138494 - 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
EEL44_RS00680 138635..138922 - 288 WP_000426470.1 hypothetical protein -
EEL44_RS00685 139074..139742 - 669 WP_000043871.1 hypothetical protein -
EEL44_RS00690 139973..140245 + 273 WP_000246253.1 DUF1778 domain-containing protein -
EEL44_RS00695 140242..140739 + 498 WP_000982260.1 GNAT family N-acetyltransferase -
EEL44_RS00705 140941..141093 - 153 WP_001884520.1 DUF645 family protein -
EEL44_RS00710 141415..141870 - 456 WP_001245327.1 GNAT family N-acetyltransferase -
EEL44_RS00715 141998..142285 - 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EEL44_RS00720 142275..142526 - 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EEL44_RS00725 142740..143153 - 414 WP_000049417.1 VOC family protein -
EEL44_RS00730 143228..143371 - 144 WP_071908341.1 DUF3265 domain-containing protein -
EEL44_RS00735 143341..143742 - 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
EEL44_RS00740 143742..144434 - 693 WP_001047169.1 GNAT family N-acetyltransferase -
EEL44_RS00745 144571..144877 - 307 Protein_138 CatB-related O-acetyltransferase -
EEL44_RS00750 144945..145925 + 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
EEL44_RS20945 146050..146205 - 156 WP_000751734.1 hypothetical protein -
EEL44_RS00755 146373..146807 - 435 WP_000256036.1 GNAT family N-acetyltransferase -
EEL44_RS00760 146944..147258 - 315 WP_000071008.1 DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 126763..233717 106954
inside Integron - - 127139..226875 99736
flank IS/Tn - - 144945..145925 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(11-71)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10991.96 Da        Isoelectric Point: 10.4934

>T113800 WP_000221354.1 NZ_CP033513:c142285-141998 [Vibrio cholerae]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Download         Length: 288 bp

>T113800 NZ_CP033513:c142285-141998 [Vibrio cholerae]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9136.29 Da        Isoelectric Point: 4.0197

>AT113800 WP_001250179.1 NZ_CP033513:c142526-142275 [Vibrio cholerae]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Download         Length: 252 bp

>AT113800 NZ_CP033513:c142526-142275 [Vibrio cholerae]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X1KZS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0X1KZS8

References