Detailed information of TA system TA046758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 116728..117361
• Replicon: chromosome
• Accession: NZ_CP007635
• Organism: Vibrio cholerae strain 2012EL-2176

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: EN18_RS14905
• Protein ID: WP_000843587.1
• Coordinates: 116728..117060 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EN18_RS14910
• Protein ID: WP_000071008.1
• Coordinates: 117047..117361 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EN18_RS21390	112169..112372	+	204	WP_001911745.1	hypothetical protein	-
EN18_RS21210	112653..112826	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
EN18_RS14880	113186..113455	+	270	WP_001198131.1	hypothetical protein	-
EN18_RS20690	113773..113944	+	172	Protein_170	DUF645 family protein	-
EN18_RS14885	114153..114539	+	387	WP_000703163.1	VOC family protein	-
EN18_RS14890	114741..114983	+	243	WP_000107461.1	hypothetical protein	-
EN18_RS14895	115154..115531	+	378	WP_000411109.1	hypothetical protein	-
EN18_RS21575	115588..115702	+	115	Protein_174	acetyltransferase	-
EN18_RS20695	115651..115932	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
EN18_RS20710	116098..116211	+	114	WP_001889158.1	hypothetical protein	-
EN18_RS20715	116160..116387	+	228	WP_073426587.1	DUF1289 domain-containing protein	-
EN18_RS14905	116728..117060	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EN18_RS14910	117047..117361	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
EN18_RS14915	117498..117932	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
EN18_RS21395	118100..118255	+	156	WP_000751734.1	hypothetical protein	-
EN18_RS14925	118380..119360	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
EN18_RS14930	119428..119734	+	307	Protein_183	CatB-related O-acetyltransferase	-
EN18_RS14935	119871..120563	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
EN18_RS14940	120563..120964	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
EN18_RS21220	120934..121077	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
EN18_RS14945	121152..121565	+	414	WP_000049417.1	VOC family protein	-
EN18_RS14950	121779..122030	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EN18_RS14955	122020..122307	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31037..137542	106505
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	36117..137166	101049
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	118380..119360	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T46758 WP_000843587.1 NZ_CP007635:116728-117060 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T46758 NZ_CP007635:116728-117060 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT46758 WP_000071008.1 NZ_CP007635:117047-117361 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT46758 NZ_CP007635:117047-117361 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA