Detailed information of TA system TA058301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 456364..457130
• Replicon: chromosome
• Accession: NZ_CP013310
• Organism: Vibrio cholerae strain E1162

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A233HKB4
• Locus tag: ASZ87_RS16425
• Protein ID: WP_000982256.1
• Coordinates: 456364..456861 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ASZ87_RS16430
• Protein ID: WP_000246254.1
• Coordinates: 456858..457130 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ87_RS16390	451764..452303	+	540	WP_053035032.1	phosphoglycerate mutase family protein	-
ASZ87_RS16395	452438..452863	+	426	WP_000693748.1	hypothetical protein	-
ASZ87_RS21055	452920..453030	+	111	WP_106746893.1	iron transporter	-
ASZ87_RS16400	453017..453376	+	360	WP_001071527.1	VOC family protein	-
ASZ87_RS21155	453518..453951	+	434	Protein_393	GNAT family N-acetyltransferase	-
ASZ87_RS16410	454088..455014	+	927	WP_000377813.1	hypothetical protein	-
ASZ87_RS20515	455337..455490	+	154	Protein_395	DUF645 family protein	-
ASZ87_RS16420	455719..456222	+	504	WP_001158950.1	DinB family protein	-
ASZ87_RS16425	456364..456861	-	498	WP_000982256.1	GNAT family N-acetyltransferase	Toxin
ASZ87_RS16430	456858..457130	-	273	WP_000246254.1	DUF1778 domain-containing protein	Antitoxin
ASZ87_RS16435	457518..457685	+	168	WP_053035038.1	DUF645 family protein	-
ASZ87_RS16440	457893..458240	+	348	WP_000933413.1	hypothetical protein	-
ASZ87_RS16450	458925..459107	+	183	WP_000923340.1	DUF645 family protein	-
ASZ87_RS16455	459322..459861	+	540	WP_000099372.1	hypothetical protein	-
ASZ87_RS16460	459984..460499	+	516	WP_000090011.1	hypothetical protein	-
ASZ87_RS16465	460634..461170	+	537	WP_000644491.1	nucleotidyltransferase family protein	-
ASZ87_RS16470	461639..461749	+	111	WP_071908335.1	DUF3265 domain-containing protein	-
ASZ87_RS16480	461962..462114	+	153	WP_001884520.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	435534..596659	161125
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	440614..596283	155669

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-89)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18539.23 Da
• Isoelectric Point: 8.7753

>T58301 WP_000982256.1 NZ_CP013310:c456861-456364 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T58301 NZ_CP013310:c456861-456364 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCATTGAAGCCCTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACACCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCTTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCGCTCAGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTCCCTGTTGTGATCGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCTGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9685.19 Da
• Isoelectric Point: 6.2988

>AT58301 WP_000246254.1 NZ_CP013310:c457130-456858 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLTKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVANAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT58301 NZ_CP013310:c457130-456858 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCACTAAGGCTGCTGCTCTTGC
TGGCATGTCTAGTATTAACTCATTTGTTTTGAATGCGGCAATTGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCGGTAGCAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A233HKB4