Detailed information of TA system TA074621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 765665..766758
• Replicon: chromosome
• Accession: NZ_CP020407
• Organism: Vibrio cholerae strain FDAARGOS_223

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: A6J62_RS03525
• Protein ID: WP_000351248.1
• Coordinates: 765665..766066 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: A6J62_RS03530
• Protein ID: WP_001047169.1
• Coordinates: 766066..766758 (-)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J62_RS03470	760959..761246	-	288	WP_000426470.1	hypothetical protein	-
A6J62_RS03475	761398..762066	-	669	WP_000043871.1	hypothetical protein	-
A6J62_RS03480	762297..762569	+	273	WP_000246253.1	DUF1778 domain-containing protein	-
A6J62_RS03485	762566..763063	+	498	WP_000982260.1	GNAT family N-acetyltransferase	-
A6J62_RS03495	763265..763417	-	153	WP_001884520.1	DUF645 family protein	-
A6J62_RS03500	763739..764194	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
A6J62_RS03505	764322..764609	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A6J62_RS03510	764599..764850	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
A6J62_RS03515	765064..765477	-	414	WP_000049417.1	VOC family protein	-
A6J62_RS03520	765552..765695	-	144	WP_071908341.1	DUF3265 domain-containing protein	-
A6J62_RS03525	765665..766066	-	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
A6J62_RS03530	766066..766758	-	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
A6J62_RS03535	766929..767201	-	273	Protein_667	CatB-related O-acetyltransferase	-
A6J62_RS03540	767269..768249	+	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
A6J62_RS19640	768374..768529	-	156	WP_000751734.1	hypothetical protein	-
A6J62_RS03545	768697..769131	-	435	WP_000256036.1	GNAT family N-acetyltransferase	-
A6J62_RS03550	769268..769582	-	315	WP_000071008.1	DNA-binding transcriptional regulator	-
A6J62_RS03555	769569..769901	-	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A6J62_RS03565	770242..770469	-	228	WP_001894457.1	DUF1289 domain-containing protein	-
A6J62_RS03570	770418..770531	-	114	WP_001889158.1	hypothetical protein	-
A6J62_RS03585	770697..770978	-	282	WP_001894453.1	DUF1289 domain-containing protein	-
A6J62_RS19645	770927..771041	-	115	Protein_676	acetyltransferase	-
A6J62_RS03590	771098..771475	-	378	WP_000411109.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	749087..881909	132822
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	749463..875103	125640
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	767269..768249	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T74621 WP_000351248.1 NZ_CP020407:c766066-765665 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T74621 NZ_CP020407:c766066-765665 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT74621 WP_001047169.1 NZ_CP020407:c766758-766066 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT74621 NZ_CP020407:c766758-766066 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34