Detailed information of TA system TA045809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 369331..369946
• Replicon: chromosome
• Accession: NZ_CP006948
• Organism: Vibrio cholerae O1 str. KW3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: N900_RS15825
• Protein ID: WP_001162670.1
• Coordinates: 369331..369618 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: N900_RS15830
• Protein ID: WP_001232701.1
• Coordinates: 369629..369946 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N900_RS20350	364967..365062	+	96	WP_001907607.1	DUF645 family protein	-
N900_RS15795	365256..365573	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N900_RS15800	365592..365834	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
N900_RS15805	366076..366585	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
N900_RS15810	366642..367295	+	654	WP_000226874.1	hypothetical protein	-
N900_RS15815	367605..367823	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
N900_RS19580	368226..368459	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
N900_RS15820	368884..369064	+	181	Protein_361	DUF645 family protein	-
N900_RS15825	369331..369618	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
N900_RS15830	369629..369946	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
N900_RS20355	369943..370053	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
N900_RS20360	370230..370355	+	126	WP_001944767.1	DUF645 family protein	-
N900_RS20365	370371..370409	+	39	WP_106019119.1	hypothetical protein	-
N900_RS15840	370657..371625	+	969	WP_016710711.1	LD-carboxypeptidase	-
N900_RS15850	372343..373272	+	930	WP_000335802.1	hypothetical protein	-
N900_RS20015	373269..373379	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
N900_RS15855	373407..373883	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
N900_RS15860	374020..374535	+	516	WP_001201528.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304645..411088	106443
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	309725..410760	101035

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T45809 WP_001162670.1 NZ_CP006948:369331-369618 [Vibrio cholerae O1 str. KW3]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T45809 NZ_CP006948:369331-369618 [Vibrio cholerae O1 str. KW3]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT45809 WP_001232701.1 NZ_CP006948:369629-369946 [Vibrio cholerae O1 str. KW3]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT45809 NZ_CP006948:369629-369946 [Vibrio cholerae O1 str. KW3]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36