58384

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 207994..208760 Replicon chromosome
Accession NZ_CP013316
Organism Vibrio cholerae strain M2140

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag ASZ83_RS15505 Protein ID WP_000982260.1
Coordinates 207994..208491 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag ASZ83_RS15510 Protein ID WP_000246253.1
Coordinates 208488..208760 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ83_RS15450 203235..204164 + 930 WP_000335802.1 hypothetical protein -
ASZ83_RS20210 204161..204271 + 111 WP_071917832.1 DUF3265 domain-containing protein -
ASZ83_RS15460 204299..204991 + 693 WP_001047177.1 GNAT family N-acetyltransferase -
ASZ83_RS15465 204991..205392 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
ASZ83_RS20215 205362..205505 + 144 WP_071908341.1 DUF3265 domain-containing protein -
ASZ83_RS15475 205580..205993 + 414 WP_000049417.1 VOC family protein -
ASZ83_RS15480 206207..206458 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ASZ83_RS15485 206448..206735 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
ASZ83_RS15490 206863..207318 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
ASZ83_RS15500 207640..207792 + 153 WP_001884520.1 DUF645 family protein -
ASZ83_RS15505 207994..208491 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
ASZ83_RS15510 208488..208760 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
ASZ83_RS15515 208991..209659 + 669 WP_000043871.1 hypothetical protein -
ASZ83_RS15520 209811..210098 + 288 WP_000426470.1 hypothetical protein -
ASZ83_RS15525 210239..210610 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
ASZ83_RS15530 210677..211102 + 426 WP_001882332.1 DUF1778 domain-containing protein -
ASZ83_RS15535 211099..211632 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
ASZ83_RS15540 211834..212091 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ASZ83_RS15545 212079..212381 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
ASZ83_RS15550 212615..213532 + 918 WP_000186333.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 87981..224211 136230
inside Integron - - 93061..221594 128533


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T58384 WP_000982260.1 NZ_CP013316:c208491-207994 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T58384 NZ_CP013316:c208491-207994 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT58384 WP_000246253.1 NZ_CP013316:c208760-208488 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT58384 NZ_CP013316:c208760-208488 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References