Detailed information of TA system TA048162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 407164..407723
• Replicon: chromosome
• Accession: NZ_CP009041
• Organism: Vibrio cholerae O1 biovar El Tor strain FJ147

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q9K2M8
• Locus tag: IR04_RS02100
• Protein ID: WP_000578476.1
• Coordinates: 407164..407442 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9L991
• Locus tag: IR04_RS02105
• Protein ID: WP_000381183.1
• Coordinates: 407439..407723 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IR04_RS02065	402464..402901	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
IR04_RS02070	402965..403183	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
IR04_RS02075	403358..404230	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
IR04_RS02080	404380..404979	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
IR04_RS20045	405036..405140	+	105	WP_099607150.1	acetyltransferase	-
IR04_RS02085	405171..405440	+	270	WP_001114075.1	DUF4160 domain-containing protein	-
IR04_RS02090	405434..405955	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
IR04_RS20050	406254..406379	+	126	WP_001905700.1	DUF645 family protein	-
IR04_RS02095	406630..407025	+	396	WP_001000867.1	hypothetical protein	-
IR04_RS02100	407164..407442	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
IR04_RS02105	407439..407723	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
IR04_RS02110	407917..408432	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
IR04_RS02115	408616..409029	+	414	WP_000049420.1	VOC family protein	-
IR04_RS02125	409584..410761	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304700..409892	105192
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	309780..409516	99736
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	402464..402901	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10819.59 Da
• Isoelectric Point: 5.1651

>T48162 WP_000578476.1 NZ_CP009041:c407442-407164 [Vibrio cholerae O1 biovar El Tor]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Nucleotide

• Length: 279 bp

>T48162 NZ_CP009041:c407442-407164 [Vibrio cholerae O1 biovar El Tor]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10901.25 Da
• Isoelectric Point: 8.0775

>AT48162 WP_000381183.1 NZ_CP009041:c407723-407439 [Vibrio cholerae O1 biovar El Tor]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Nucleotide

• Length: 285 bp

>AT48162 NZ_CP009041:c407723-407439 [Vibrio cholerae O1 biovar El Tor]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: Q9K2M8

Antitoxin

• AlphaFold DB: A0A067BL33