64440

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-GNAT
Location 436911..438004 Replicon chromosome
Accession NZ_CP016325
Organism Vibrio cholerae 2740-80

Toxin (Protein)


Gene name doc Uniprot ID Q9KMA2
Locus tag BBB50_RS16160 Protein ID WP_000351248.1
Coordinates 437603..438004 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID -
Locus tag BBB50_RS16155 Protein ID WP_001047169.1
Coordinates 436911..437603 (+) Length 231 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBB50_RS16110 432194..432571 + 378 WP_000411109.1 hypothetical protein -
BBB50_RS20540 432628..432742 + 115 Protein_456 acetyltransferase -
BBB50_RS16115 432691..432972 + 282 WP_001894453.1 DUF1289 domain-containing protein -
BBB50_RS19980 433138..433251 + 114 WP_001889158.1 hypothetical protein -
BBB50_RS19985 433200..433427 + 228 WP_001894457.1 DUF1289 domain-containing protein -
BBB50_RS16125 433768..434100 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin -
BBB50_RS16130 434087..434401 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
BBB50_RS16135 434538..434972 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
BBB50_RS16140 435140..435295 + 156 WP_000751734.1 hypothetical protein -
BBB50_RS16145 435420..436400 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
BBB50_RS16150 436468..436774 + 307 Protein_465 CatB-related O-acetyltransferase -
BBB50_RS16155 436911..437603 + 693 WP_001047169.1 GNAT family N-acetyltransferase Antitoxin
BBB50_RS16160 437603..438004 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
BBB50_RS20340 437974..438117 + 144 WP_071908341.1 DUF3265 domain-containing protein -
BBB50_RS16165 438192..438605 + 414 WP_000049417.1 VOC family protein -
BBB50_RS16170 438819..439070 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BBB50_RS16175 439060..439347 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
BBB50_RS16180 439475..439930 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
BBB50_RS20000 440252..440404 + 153 WP_001884520.1 DUF645 family protein -
BBB50_RS16185 440606..441103 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
BBB50_RS16190 441100..441372 - 273 WP_000246253.1 DUF1778 domain-containing protein -
BBB50_RS16195 441603..442271 + 669 WP_000043871.1 hypothetical protein -
BBB50_RS16200 442423..442710 + 288 WP_000426470.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 304243..451810 147567
- inside Integron - - 309323..451434 142111
flank IS/Tn - - 435420..436400 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-132)

Antitoxin

GNAT domain

(36-153)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14608.71 Da        Isoelectric Point: 4.0128

>T64440 WP_000351248.1 NZ_CP016325:437603-438004 [Vibrio cholerae 2740-80]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Download         Length: 402 bp

>T64440 NZ_CP016325:437603-438004 [Vibrio cholerae 2740-80]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin


Download         Length: 231 a.a.        Molecular weight: 26352.73 Da        Isoelectric Point: 8.5633

>AT64440 WP_001047169.1 NZ_CP016325:436911-437603 [Vibrio cholerae 2740-80]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Download         Length: 693 bp

>AT64440 NZ_CP016325:436911-437603 [Vibrio cholerae 2740-80]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0PN34

Antitoxin

Source ID Structure

References