Detailed information of TA system TA056922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-Phd
• Location: 389756..390303
• Replicon: chromosome
• Accession: NZ_CP012666
• Organism: Vibrio cholerae strain ICDC-VC661

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q9KM92
• Locus tag: AN947_RS02260
• Protein ID: WP_000229317.1
• Coordinates: 390001..390303 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KM93
• Locus tag: AN947_RS02255
• Protein ID: WP_000861987.1
• Coordinates: 389756..390013 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN947_RS02190	384785..385240	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
AN947_RS02200	385562..385714	+	153	WP_001884520.1	DUF645 family protein	-
AN947_RS02210	385916..386413	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
AN947_RS02215	386410..386682	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
AN947_RS02225	386913..387581	+	669	WP_000043871.1	hypothetical protein	-
AN947_RS02230	387733..388020	+	288	WP_000426470.1	hypothetical protein	-
AN947_RS02235	388161..388532	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
AN947_RS20785	388599..389024	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
AN947_RS02245	389021..389554	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
AN947_RS02255	389756..390013	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AN947_RS02260	390001..390303	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AN947_RS02270	390537..391454	+	918	WP_000186333.1	alpha/beta hydrolase	-
AN947_RS02280	391611..392000	+	390	WP_001081302.1	hypothetical protein	-
AN947_RS02285	392136..392345	+	210	Protein_407	GNAT family N-acetyltransferase	-
AN947_RS02290	392464..392901	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
AN947_RS02295	392965..393183	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
AN947_RS02300	393358..394230	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
AN947_RS02305	394380..394979	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
AN947_RS20790	395036..395140	+	105	WP_099607150.1	acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304645..399892	95247
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309725..399516	89791
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	392464..392901	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11726.68 Da
• Isoelectric Point: 5.1972

>T56922 WP_000229317.1 NZ_CP012666:390001-390303 [Vibrio cholerae]
MVEIIWTELALSDLNDIAEYIALENVVAAKQLVQTVFTKVERLADFPESGRVPPELEHLNYREVVVSPCRVFYKYDDAKV
RILFVMRAERDLRRLMLTKQ

Nucleotide

• Length: 303 bp

>T56922 NZ_CP012666:390001-390303 [Vibrio cholerae]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAGTCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9647.15 Da
• Isoelectric Point: 7.3178

>AT56922 WP_000861987.1 NZ_CP012666:389756-390013 [Vibrio cholerae]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Nucleotide

• Length: 258 bp

>AT56922 NZ_CP012666:389756-390013 [Vibrio cholerae]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Structures

Toxin

• AlphaFold DB: Q9KM92

Antitoxin

• AlphaFold DB: A0A1T4SLM9