Detailed information of TA system TA104256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 417259..417892
• Replicon: chromosome
• Accession: NZ_CP028828
• Organism: Vibrio cholerae strain N16961

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: N16961_RS16520
• Protein ID: WP_000843587.1
• Coordinates: 417259..417591 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: N16961_RS16525
• Protein ID: WP_000071008.1
• Coordinates: 417578..417892 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N16961_RS19765	412700..412903	+	204	WP_001911745.1	hypothetical protein	-
N16961_RS16450	413184..413357	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
N16961_RS16455	413717..413986	+	270	WP_001198131.1	hypothetical protein	-
N16961_RS19940	414304..414475	+	172	Protein_435	DUF645 family protein	-
N16961_RS16470	414684..415070	+	387	WP_000703163.1	VOC family protein	-
N16961_RS16475	415272..415514	+	243	WP_000107461.1	hypothetical protein	-
N16961_RS16480	415685..416062	+	378	WP_000411109.1	hypothetical protein	-
N16961_RS19945	416119..416233	+	115	Protein_439	acetyltransferase	-
N16961_RS16485	416182..416463	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
N16961_RS16500	416629..416742	+	114	WP_001889158.1	hypothetical protein	-
N16961_RS16505	416691..416918	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
N16961_RS16520	417259..417591	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
N16961_RS16525	417578..417892	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
N16961_RS16530	418029..418463	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
N16961_RS19670	418631..418786	+	156	WP_000751734.1	hypothetical protein	-
N16961_RS16535	418911..419891	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
N16961_RS16540	419959..420265	+	307	Protein_448	CatB-related O-acetyltransferase	-
N16961_RS16545	420402..421094	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
N16961_RS16550	421094..421495	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
N16961_RS16555	421465..421608	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
N16961_RS16560	421683..422096	+	414	WP_000049417.1	VOC family protein	-
N16961_RS16565	422310..422561	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
N16961_RS16570	422551..422838	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306977..438073	131096
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	312057..437697	125640
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	418911..419891	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T104256 WP_000843587.1 NZ_CP028828:417259-417591 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T104256 NZ_CP028828:417259-417591 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT104256 WP_000071008.1 NZ_CP028828:417578-417892 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT104256 NZ_CP028828:417578-417892 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA