125042

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 936034..936649 Replicon chromosome
Accession NZ_CP040173
Organism Vibrio cholerae strain VC1374

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag FDZ90_RS18990 Protein ID WP_001162670.1
Coordinates 936034..936321 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag FDZ90_RS18995 Protein ID WP_001232701.1
Coordinates 936332..936649 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FDZ90_RS18950 931670..931765 + 96 WP_001907607.1 DUF645 family protein -
FDZ90_RS18955 931959..932276 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
FDZ90_RS18960 932295..932537 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FDZ90_RS18965 932779..933288 + 510 WP_000405987.1 GNAT family N-acetyltransferase -
FDZ90_RS18970 933345..933998 + 654 WP_000226874.1 hypothetical protein -
FDZ90_RS18975 934308..934526 + 219 WP_001917086.1 DUF3709 domain-containing protein -
FDZ90_RS18980 934929..935162 + 234 WP_032482776.1 DUF3709 domain-containing protein -
FDZ90_RS18985 935587..935767 + 181 Protein_850 DUF645 family protein -
FDZ90_RS18990 936034..936321 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FDZ90_RS18995 936332..936649 + 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
FDZ90_RS19000 936646..936756 + 111 WP_134814058.1 DUF3265 domain-containing protein -
FDZ90_RS19005 936933..937058 + 126 WP_001944767.1 DUF645 family protein -
FDZ90_RS19010 937074..937112 + 39 WP_106019119.1 hypothetical protein -
FDZ90_RS19015 937325..938329 + 1005 WP_000964925.1 LD-carboxypeptidase -
FDZ90_RS19020 938482..938928 + 447 WP_000128977.1 hypothetical protein -
FDZ90_RS19025 939048..939977 + 930 WP_000335802.1 hypothetical protein -
FDZ90_RS19030 939974..940084 + 111 WP_071917832.1 DUF3265 domain-containing protein -
FDZ90_RS19035 940112..940588 + 477 WP_001047180.1 GNAT family N-acetyltransferase -
FDZ90_RS19040 940725..941240 + 516 WP_001201528.1 lipocalin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 882589..977840 95251
inside Integron catB9 - 887669..977464 89795


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T125042 WP_001162670.1 NZ_CP040173:936034-936321 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T125042 NZ_CP040173:936034-936321 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT125042 WP_001232701.1 NZ_CP040173:936332-936649 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT125042 NZ_CP040173:936332-936649 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References