58251

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 413698..414464 Replicon chromosome
Accession NZ_CP013304
Organism Vibrio cholerae strain CRC711

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag ASZ82_RS16600 Protein ID WP_000982260.1
Coordinates 413698..414195 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag ASZ82_RS16605 Protein ID WP_000246253.1
Coordinates 414192..414464 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ82_RS16550 409560..409866 + 307 Protein_429 CatB-related O-acetyltransferase -
ASZ82_RS16555 410003..410695 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
ASZ82_RS16560 410695..411096 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
ASZ82_RS20175 411066..411209 + 144 WP_071908341.1 DUF3265 domain-containing protein -
ASZ82_RS16570 411284..411697 + 414 WP_000049417.1 VOC family protein -
ASZ82_RS16575 411911..412162 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ASZ82_RS16580 412152..412439 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
ASZ82_RS16585 412567..413022 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
ASZ82_RS16595 413344..413496 + 153 WP_001884520.1 DUF645 family protein -
ASZ82_RS16600 413698..414195 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
ASZ82_RS16605 414192..414464 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
ASZ82_RS16610 414695..415363 + 669 WP_000043871.1 hypothetical protein -
ASZ82_RS16615 415515..415802 + 288 WP_000426470.1 hypothetical protein -
ASZ82_RS16620 415943..416314 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
ASZ82_RS16625 416381..416806 + 426 WP_001882332.1 DUF1778 domain-containing protein -
ASZ82_RS16630 416803..417336 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
ASZ82_RS16635 417538..417795 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ASZ82_RS16640 417783..418085 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
ASZ82_RS16645 418319..419236 + 918 WP_000186333.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304736..427674 122938
- inside Integron - - 309816..427298 117482
flank IS/Tn - - 408512..409492 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T58251 WP_000982260.1 NZ_CP013304:c414195-413698 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T58251 NZ_CP013304:c414195-413698 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT58251 WP_000246253.1 NZ_CP013304:c414464-414192 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT58251 NZ_CP013304:c414464-414192 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References