Detailed information of TA system TA046752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 60257..60755
• Replicon: chromosome
• Accession: NZ_CP007635
• Organism: Vibrio cholerae strain 2012EL-2176

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: EN18_RS14485
• Protein ID: WP_000589156.1
• Coordinates: 60257..60523 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: EN18_RS14490
• Protein ID: WP_000643598.1
• Coordinates: 60510..60755 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EN18_RS21105	55678..55884	+	207	Protein_69	DUF3709 domain-containing protein	-
EN18_RS21110	56084..56185	+	102	WP_001921603.1	hypothetical protein	-
EN18_RS14450	56175..56561	+	387	WP_000703163.1	VOC family protein	-
EN18_RS14455	56756..57007	+	252	WP_001890111.1	TIGR03643 family protein	-
EN18_RS20520	56980..57129	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
EN18_RS14460	57221..57463	+	243	WP_000107461.1	hypothetical protein	-
EN18_RS14465	57715..57993	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
EN18_RS21540	58368..58511	+	144	Protein_76	DUF645 family protein	-
EN18_RS21545	58565..58603	+	39	WP_082798268.1	hypothetical protein	-
EN18_RS14475	58815..59282	+	468	WP_001289288.1	OsmC family protein	-
EN18_RS14480	59410..60072	+	663	WP_000960654.1	hypothetical protein	-
EN18_RS14485	60257..60523	+	267	WP_000589156.1	BrnT family toxin	Toxin
EN18_RS14490	60510..60755	+	246	WP_000643598.1	hypothetical protein	Antitoxin
EN18_RS14495	60851..61645	+	795	WP_001911581.1	hypothetical protein	-
EN18_RS21550	61748..61876	+	129	WP_071908338.1	general secretion pathway protein GspI	-
EN18_RS14500	61937..62119	+	183	WP_000923182.1	DUF645 family protein	-
EN18_RS14505	62335..63339	+	1005	WP_000964920.1	LD-carboxypeptidase	-
EN18_RS14510	63492..63851	+	360	WP_001071541.1	VOC family protein	-
EN18_RS14515	64124..64357	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
EN18_RS14520	64625..65131	+	507	WP_000393074.1	hypothetical protein	-
EN18_RS14525	65288..65674	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31037..137542	106505
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	36117..137166	101049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T46752 WP_000589156.1 NZ_CP007635:60257-60523 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T46752 NZ_CP007635:60257-60523 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT46752 WP_000643598.1 NZ_CP007635:60510-60755 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT46752 NZ_CP007635:60510-60755 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2