26154

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 419819..420347 Replicon chromosome II
Accession NC_016945
Organism Vibrio cholerae IEC224

Toxin (Protein)


Gene name relE Uniprot ID A0A0X1KZS6
Locus tag O3Y_RS16175 Protein ID WP_000221354.1
Coordinates 420060..420347 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X1KZS8
Locus tag O3Y_RS16170 Protein ID WP_001250179.1
Coordinates 419819..420070 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3Y_RS16130 415087..415401 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
O3Y_RS16135 415538..415972 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
O3Y_RS20420 416140..416295 + 156 WP_000751734.1 hypothetical protein -
O3Y_RS16145 416420..417400 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
O3Y_RS16150 417468..417774 + 307 Protein_444 CatB-related O-acetyltransferase -
O3Y_RS16155 417911..418603 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
O3Y_RS16160 418603..419004 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
O3Y_RS20335 418974..419117 + 144 WP_071908341.1 DUF3265 domain-containing protein -
O3Y_RS16165 419192..419605 + 414 WP_000049417.1 VOC family protein -
O3Y_RS16170 419819..420070 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
O3Y_RS16175 420060..420347 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
O3Y_RS16180 420475..420930 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
O3Y_RS16185 421252..421404 + 153 WP_001884520.1 DUF645 family protein -
O3Y_RS16190 421606..422103 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
O3Y_RS16195 422100..422372 - 273 WP_000246253.1 DUF1778 domain-containing protein -
O3Y_RS16200 422603..423271 + 669 WP_000043871.1 hypothetical protein -
O3Y_RS16205 423423..423710 + 288 WP_000426470.1 hypothetical protein -
O3Y_RS16210 423851..424222 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
O3Y_RS16215 424289..424714 + 426 WP_001882332.1 DUF1778 domain-containing protein -
O3Y_RS16220 424711..425244 + 534 WP_000118351.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304637..435582 130945
inside Integron - - 309717..435206 125489
flank IS/Tn - - 416420..417400 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(11-71)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10991.96 Da        Isoelectric Point: 10.4934

>T26154 WP_000221354.1 NC_016945:420060-420347 [Vibrio cholerae IEC224]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Download         Length: 288 bp

>T26154 NC_016945:420060-420347 [Vibrio cholerae IEC224]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9136.29 Da        Isoelectric Point: 4.0197

>AT26154 WP_001250179.1 NC_016945:419819-420070 [Vibrio cholerae IEC224]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Download         Length: 252 bp

>AT26154 NC_016945:419819-420070 [Vibrio cholerae IEC224]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X1KZS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0X1KZS8

References