58408

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-Phd
Location 486697..487244 Replicon chromosome
Accession NZ_CP013318
Organism Vibrio cholerae strain NCTC5395

Toxin (Protein)


Gene name relE Uniprot ID Q9KM92
Locus tag ASZ85_RS17135 Protein ID WP_000229317.1
Coordinates 486942..487244 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9KM93
Locus tag ASZ85_RS17130 Protein ID WP_000861987.1
Coordinates 486697..486954 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ85_RS17080 481726..482181 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
ASZ85_RS17090 482503..482655 + 153 WP_001884520.1 DUF645 family protein -
ASZ85_RS17095 482857..483354 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
ASZ85_RS17100 483351..483623 - 273 WP_000246253.1 DUF1778 domain-containing protein -
ASZ85_RS17105 483854..484522 + 669 WP_000043871.1 hypothetical protein -
ASZ85_RS17110 484674..484961 + 288 WP_000426470.1 hypothetical protein -
ASZ85_RS17115 485102..485473 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
ASZ85_RS17120 485540..485965 + 426 WP_001882332.1 DUF1778 domain-containing protein -
ASZ85_RS17125 485962..486495 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
ASZ85_RS17130 486697..486954 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ASZ85_RS17135 486942..487244 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ASZ85_RS17140 487478..488395 + 918 WP_000186333.1 alpha/beta hydrolase -
ASZ85_RS17150 488552..488941 + 390 WP_001081302.1 hypothetical protein -
ASZ85_RS17155 489077..489286 + 210 Protein_549 GNAT family N-acetyltransferase -
ASZ85_RS17160 489405..489842 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
ASZ85_RS17165 489906..490124 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
ASZ85_RS17175 490299..491171 + 873 WP_000254716.1 DUF3800 domain-containing protein -
ASZ85_RS17180 491321..491920 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
ASZ85_RS21230 491977..492081 + 105 WP_099607150.1 acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 338285..496833 158548
inside Integron - - 343365..496457 153092
flank IS/Tn - - 489405..489842 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-69)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11726.68 Da        Isoelectric Point: 5.1972

>T58408 WP_000229317.1 NZ_CP013318:486942-487244 [Vibrio cholerae]
MVEIIWTELALSDLNDIAEYIALENVVAAKQLVQTVFTKVERLADFPESGRVPPELEHLNYREVVVSPCRVFYKYDDAKV
RILFVMRAERDLRRLMLTKQ

Download         Length: 303 bp

>T58408 NZ_CP013318:486942-487244 [Vibrio cholerae]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAGTCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9647.15 Da        Isoelectric Point: 7.3178

>AT58408 WP_000861987.1 NZ_CP013318:486697-486954 [Vibrio cholerae]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Download         Length: 258 bp

>AT58408 NZ_CP013318:486697-486954 [Vibrio cholerae]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9KM92

Antitoxin

Source ID Structure
AlphaFold DB A0A1T4SLM9

References