58393

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-Phd
Location 372121..372699 Replicon chromosome
Accession NZ_CP013318
Organism Vibrio cholerae strain NCTC5395

Toxin (Protein)


Gene name parE Uniprot ID O68848
Locus tag ASZ85_RS15950 Protein ID WP_001180243.1
Coordinates 372121..372438 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID Q7DCR7
Locus tag ASZ85_RS15955 Protein ID WP_000557292.1
Coordinates 372457..372699 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ85_RS15920 367645..367827 + 183 WP_000923340.1 DUF645 family protein -
ASZ85_RS15925 368181..368363 + 183 WP_000923153.1 DUF645 family protein -
ASZ85_RS15930 368558..369235 + 678 WP_000254617.1 HNH endonuclease -
ASZ85_RS15935 369397..370605 + 1209 WP_001900186.1 deoxyguanosinetriphosphate triphosphohydrolase family protein -
ASZ85_RS15940 370767..371471 + 705 WP_000087610.1 HNH endonuclease -
ASZ85_RS15945 371629..371967 + 339 WP_000713853.1 hypothetical protein -
ASZ85_RS15950 372121..372438 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ASZ85_RS15955 372457..372699 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ASZ85_RS15960 372943..373329 + 387 WP_001015867.1 NUDIX hydrolase -
ASZ85_RS15965 373386..373499 + 114 WP_001900214.1 hypothetical protein -
ASZ85_RS15970 373448..373729 + 282 WP_001894429.1 DUF1289 domain-containing protein -
ASZ85_RS15980 373987..374523 + 537 WP_000469482.1 GNAT family N-acetyltransferase -
ASZ85_RS15985 374660..375175 + 516 WP_001201509.1 lipocalin family protein -
ASZ85_RS15990 375325..375822 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
ASZ85_RS15995 375819..376091 - 273 WP_000246253.1 DUF1778 domain-containing protein -
ASZ85_RS20980 376484..376609 + 126 WP_001900195.1 DUF645 family protein -
ASZ85_RS20985 376625..376663 + 39 WP_106019118.1 hypothetical protein -
ASZ85_RS16005 376859..377305 + 447 WP_000006157.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 338285..496833 158548
inside Integron - - 343365..496457 153092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-93)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-71)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12158.97 Da        Isoelectric Point: 9.7495

>T58393 WP_001180243.1 NZ_CP013318:c372438-372121 [Vibrio cholerae]
MQNKQYKLSQLAQEHLLKIKHYTIENFAEAQWQKYKSTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS

Download         Length: 318 bp

>T58393 NZ_CP013318:c372438-372121 [Vibrio cholerae]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8961.25 Da        Isoelectric Point: 5.6630

>AT58393 WP_000557292.1 NZ_CP013318:c372699-372457 [Vibrio cholerae]
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDSGKTVDGATFLNTL

Download         Length: 243 bp

>AT58393 NZ_CP013318:c372699-372457 [Vibrio cholerae]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K9UJR8

Antitoxin

Source ID Structure
AlphaFold DB Q7DCR7

References