Detailed information of TA system TA025718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 125011..125966
• Replicon: chromosome
• Accession: NC_016446
• Organism: Vibrio cholerae O1 str. 2010EL-1786

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: HJ37_RS14850
• Protein ID: WP_000118351.1
• Coordinates: 125433..125966 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HJ37_RS20245
• Protein ID: WP_001882332.1
• Coordinates: 125011..125436 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ37_RS14800	120541..120792	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HJ37_RS14805	120782..121069	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HJ37_RS14810	121197..121652	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
HJ37_RS14815	121974..122126	+	153	WP_001884520.1	DUF645 family protein	-
HJ37_RS14820	122328..122825	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
HJ37_RS14825	122822..123094	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
HJ37_RS14830	123325..123993	+	669	WP_000043871.1	hypothetical protein	-
HJ37_RS14835	124145..124432	+	288	WP_000426470.1	hypothetical protein	-
HJ37_RS14840	124573..124944	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
HJ37_RS20245	125011..125436	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
HJ37_RS14850	125433..125966	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
HJ37_RS14855	126168..126425	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HJ37_RS14860	126413..126715	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HJ37_RS14865	126949..127866	+	918	WP_000186333.1	alpha/beta hydrolase	-
HJ37_RS14870	128023..128412	+	390	WP_001081302.1	hypothetical protein	-
HJ37_RS14875	128548..128757	+	210	Protein_202	GNAT family N-acetyltransferase	-
HJ37_RS14880	128876..129313	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
HJ37_RS14885	129377..129595	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
HJ37_RS14890	129770..130642	+	873	WP_000254716.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31115..136304	105189
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	catB9	-	36195..135928	99733
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	128876..129313	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T25718 WP_000118351.1 NC_016446:125433-125966 [Vibrio cholerae O1 str. 2010EL-1786]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T25718 NC_016446:125433-125966 [Vibrio cholerae O1 str. 2010EL-1786]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT25718 WP_001882332.1 NC_016446:125011-125436 [Vibrio cholerae O1 str. 2010EL-1786]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT25718 NC_016446:125011-125436 [Vibrio cholerae O1 str. 2010EL-1786]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94