Detailed information of TA system TA058348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 325530..326296
• Replicon: chromosome
• Accession: NZ_CP013314
• Organism: Vibrio cholerae strain E9120

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A233HKB4
• Locus tag: ASZ79_RS15640
• Protein ID: WP_000982256.1
• Coordinates: 325530..326027 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ASZ79_RS15645
• Protein ID: WP_000246254.1
• Coordinates: 326024..326296 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ79_RS15605	320930..321469	+	540	WP_053035032.1	phosphoglycerate mutase family protein	-
ASZ79_RS15610	321604..322029	+	426	WP_000693748.1	hypothetical protein	-
ASZ79_RS20975	322086..322196	+	111	WP_106746893.1	iron transporter	-
ASZ79_RS15615	322183..322542	+	360	WP_001071527.1	VOC family protein	-
ASZ79_RS21075	322684..323117	+	434	Protein_277	GNAT family N-acetyltransferase	-
ASZ79_RS15625	323254..324180	+	927	WP_000377813.1	hypothetical protein	-
ASZ79_RS20435	324503..324656	+	154	Protein_279	DUF645 family protein	-
ASZ79_RS15635	324885..325388	+	504	WP_001158950.1	DinB family protein	-
ASZ79_RS15640	325530..326027	-	498	WP_000982256.1	GNAT family N-acetyltransferase	Toxin
ASZ79_RS15645	326024..326296	-	273	WP_000246254.1	DUF1778 domain-containing protein	Antitoxin
ASZ79_RS15650	326684..326851	+	168	WP_053035038.1	DUF645 family protein	-
ASZ79_RS15655	327059..327406	+	348	WP_000933413.1	hypothetical protein	-
ASZ79_RS15665	328091..328273	+	183	WP_000923340.1	DUF645 family protein	-
ASZ79_RS15670	328488..329027	+	540	WP_000099372.1	hypothetical protein	-
ASZ79_RS15675	329150..329665	+	516	WP_000090011.1	hypothetical protein	-
ASZ79_RS15680	329800..330336	+	537	WP_000644491.1	nucleotidyltransferase family protein	-
ASZ79_RS15685	330805..330915	+	111	WP_071908335.1	DUF3265 domain-containing protein	-
ASZ79_RS15695	331128..331280	+	153	WP_001884520.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304700..468464	163764
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309780..468088	158308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-89)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18539.23 Da
• Isoelectric Point: 8.7753

>T58348 WP_000982256.1 NZ_CP013314:c326027-325530 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T58348 NZ_CP013314:c326027-325530 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCATTGAAGCCCTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACACCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCTTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCGCTCAGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTCCCTGTTGTGATCGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCTGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9685.19 Da
• Isoelectric Point: 6.2988

>AT58348 WP_000246254.1 NZ_CP013314:c326296-326024 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLTKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVANAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT58348 NZ_CP013314:c326296-326024 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCACTAAGGCTGCTGCTCTTGC
TGGCATGTCTAGTATTAACTCATTTGTTTTGAATGCGGCAATTGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCGGTAGCAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A233HKB4