64442

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 440606..441372 Replicon chromosome
Accession NZ_CP016325
Organism Vibrio cholerae 2740-80

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag BBB50_RS16185 Protein ID WP_000982260.1
Coordinates 440606..441103 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag BBB50_RS16190 Protein ID WP_000246253.1
Coordinates 441100..441372 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBB50_RS16150 436468..436774 + 307 Protein_465 CatB-related O-acetyltransferase -
BBB50_RS16155 436911..437603 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
BBB50_RS16160 437603..438004 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
BBB50_RS20340 437974..438117 + 144 WP_071908341.1 DUF3265 domain-containing protein -
BBB50_RS16165 438192..438605 + 414 WP_000049417.1 VOC family protein -
BBB50_RS16170 438819..439070 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BBB50_RS16175 439060..439347 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
BBB50_RS16180 439475..439930 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
BBB50_RS20000 440252..440404 + 153 WP_001884520.1 DUF645 family protein -
BBB50_RS16185 440606..441103 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
BBB50_RS16190 441100..441372 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
BBB50_RS16195 441603..442271 + 669 WP_000043871.1 hypothetical protein -
BBB50_RS16200 442423..442710 + 288 WP_000426470.1 hypothetical protein -
BBB50_RS16205 442851..443222 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
BBB50_RS20545 443289..443714 + 426 WP_001882332.1 DUF1778 domain-containing protein -
BBB50_RS16215 443711..444244 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
BBB50_RS16220 444446..444703 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BBB50_RS16225 444691..444993 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
BBB50_RS16230 445264..446148 + 885 WP_000842472.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 304243..451810 147567
- inside Integron - - 309323..451434 142111
flank IS/Tn - - 435420..436400 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T64442 WP_000982260.1 NZ_CP016325:c441103-440606 [Vibrio cholerae 2740-80]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T64442 NZ_CP016325:c441103-440606 [Vibrio cholerae 2740-80]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT64442 WP_000246253.1 NZ_CP016325:c441372-441100 [Vibrio cholerae 2740-80]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT64442 NZ_CP016325:c441372-441100 [Vibrio cholerae 2740-80]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References