Detailed information of TA system TA058382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 204299..205392
• Replicon: chromosome
• Accession: NZ_CP013316
• Organism: Vibrio cholerae strain M2140

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: ASZ83_RS15465
• Protein ID: WP_000351248.1
• Coordinates: 204991..205392 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: ASZ83_RS15460
• Protein ID: WP_001047177.1
• Coordinates: 204299..204991 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ83_RS15415	199935..200114	+	180	WP_001889238.1	DUF645 family protein	-
ASZ83_RS15420	200308..200823	+	516	WP_000343784.1	GNAT family N-acetyltransferase	-
ASZ83_RS20480	201120..201245	+	126	WP_001944767.1	DUF645 family protein	-
ASZ83_RS20485	201261..201299	+	39	WP_106019119.1	hypothetical protein	-
ASZ83_RS15440	201512..202516	+	1005	WP_000964925.1	LD-carboxypeptidase	-
ASZ83_RS15445	202669..203115	+	447	WP_000128977.1	hypothetical protein	-
ASZ83_RS15450	203235..204164	+	930	WP_000335802.1	hypothetical protein	-
ASZ83_RS20210	204161..204271	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
ASZ83_RS15460	204299..204991	+	693	WP_001047177.1	GNAT family N-acetyltransferase	Antitoxin
ASZ83_RS15465	204991..205392	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ASZ83_RS20215	205362..205505	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ83_RS15475	205580..205993	+	414	WP_000049417.1	VOC family protein	-
ASZ83_RS15480	206207..206458	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ83_RS15485	206448..206735	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ83_RS15490	206863..207318	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ83_RS15500	207640..207792	+	153	WP_001884520.1	DUF645 family protein	-
ASZ83_RS15505	207994..208491	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ83_RS15510	208488..208760	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ83_RS15515	208991..209659	+	669	WP_000043871.1	hypothetical protein	-
ASZ83_RS15520	209811..210098	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	87981..224211	136230
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.008	inside	Integron	-	-	93061..221594	128533

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T58382 WP_000351248.1 NZ_CP013316:204991-205392 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T58382 NZ_CP013316:204991-205392 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26306.64 Da
• Isoelectric Point: 8.6819

>AT58382 WP_001047177.1 NZ_CP013316:204299-204991 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGGPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT58382 NZ_CP013316:204299-204991 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTGGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34