Detailed information of TA system TA058313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 533788..534403
• Replicon: chromosome
• Accession: NZ_CP013310
• Organism: Vibrio cholerae strain E1162

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: ASZ87_RS17195
• Protein ID: WP_001162670.1
• Coordinates: 533788..534075 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ87_RS17200
• Protein ID: WP_001232701.1
• Coordinates: 534086..534403 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ87_RS21085	529424..529519	+	96	WP_001907607.1	DUF645 family protein	-
ASZ87_RS17155	529713..530030	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ87_RS17160	530049..530291	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ87_RS17165	530533..531042	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
ASZ87_RS17170	531099..531752	+	654	WP_000226874.1	hypothetical protein	-
ASZ87_RS17175	532062..532280	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
ASZ87_RS17185	532683..532916	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
ASZ87_RS17190	533341..533521	+	181	Protein_534	DUF645 family protein	-
ASZ87_RS17195	533788..534075	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ87_RS17200	534086..534403	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
ASZ87_RS21090	534400..534510	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
ASZ87_RS21095	534687..534812	+	126	WP_001944767.1	DUF645 family protein	-
ASZ87_RS21100	534828..534866	+	39	WP_106019119.1	hypothetical protein	-
ASZ87_RS17220	535090..535830	+	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
ASZ87_RS17225	536035..536865	+	831	WP_000056616.1	abortive infection family protein	-
ASZ87_RS17230	536922..537404	+	483	WP_001895003.1	hypothetical protein	-
ASZ87_RS17240	537851..538726	+	876	WP_000259044.1	hypothetical protein	-
ASZ87_RS17245	538856..539311	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	435534..596659	161125
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	440614..596283	155669

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T58313 WP_001162670.1 NZ_CP013310:533788-534075 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T58313 NZ_CP013310:533788-534075 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT58313 WP_001232701.1 NZ_CP013310:534086-534403 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT58313 NZ_CP013310:534086-534403 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36