Detailed information of TA system TA230521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 358082..358697
• Replicon: chromosome
• Accession: NZ_CP090379
• Organism: Vibrio cholerae strain BY369

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: LVJ30_RS15620
• Protein ID: WP_001162670.1
• Coordinates: 358082..358369 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LVJ30_RS15625
• Protein ID: WP_001232701.1
• Coordinates: 358380..358697 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ30_RS15560 (LVJ30_15565)	353425..353600	+	176	Protein_345	acetyltransferase	-
LVJ30_RS15565 (LVJ30_15570)	353661..353813	+	153	Protein_346	DUF645 family protein	-
LVJ30_RS15570 (LVJ30_15575)	354007..354324	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LVJ30_RS15575 (LVJ30_15580)	354343..354585	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LVJ30_RS15580 (LVJ30_15585)	354827..355336	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
LVJ30_RS15585 (LVJ30_15590)	355393..356046	+	654	WP_000226874.1	hypothetical protein	-
LVJ30_RS15590 (LVJ30_15595)	356135..356344	+	210	WP_001900246.1	DUF3709 domain-containing protein	-
LVJ30_RS15595 (LVJ30_15600)	356356..356574	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
LVJ30_RS15600 (LVJ30_15605)	356763..356956	+	194	Protein_353	hypothetical protein	-
LVJ30_RS15605 (LVJ30_15610)	356977..357210	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
LVJ30_RS15610 (LVJ30_15615)	357635..357815	+	181	Protein_355	DUF645 family protein	-
LVJ30_RS15615 (LVJ30_15620)	357928..358041	+	114	WP_001894955.1	hypothetical protein	-
LVJ30_RS15620 (LVJ30_15625)	358082..358369	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ30_RS15625 (LVJ30_15630)	358380..358697	+	318	WP_001232701.1	HigA family addiction module antitoxin	Antitoxin
LVJ30_RS15630 (LVJ30_15635)	358694..358804	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
LVJ30_RS15635 (LVJ30_15640)	358843..358953	+	111	Protein_360	acetyltransferase	-
LVJ30_RS15640 (LVJ30_15645)	358981..359106	+	126	WP_001944767.1	DUF645 family protein	-
LVJ30_RS15645 (LVJ30_15650)	359069..359185	+	117	WP_001889170.1	hypothetical protein	-
LVJ30_RS15650 (LVJ30_15655)	359373..360377	+	1005	WP_000964925.1	LD-carboxypeptidase	-
LVJ30_RS15655 (LVJ30_15660)	360530..360976	+	447	WP_000128977.1	hypothetical protein	-
LVJ30_RS15660 (LVJ30_15665)	361096..362025	+	930	WP_000335802.1	hypothetical protein	-
LVJ30_RS15665 (LVJ30_15670)	362022..362132	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
LVJ30_RS15670 (LVJ30_15675)	362160..362636	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
LVJ30_RS15675 (LVJ30_15680)	362827..363288	+	462	WP_001903091.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304645..399892	95247
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309725..399516	89791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T230521 WP_001162670.1 NZ_CP090379:358082-358369 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T230521 NZ_CP124916:c2797458-2797117 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT230521 WP_001232701.1 NZ_CP090379:358380-358697 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT230521 NZ_CP124916:c2797688-2797458 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36