74615

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DhiAT/-
Location 753024..753808 Replicon chromosome
Accession NZ_CP020407
Organism Vibrio cholerae strain FDAARGOS_223

Toxin (Protein)


Gene name dhiT Uniprot ID A0A086SLD6
Locus tag A6J62_RS03395 Protein ID WP_001114075.1
Coordinates 753539..753808 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name dhiA Uniprot ID Q9KM84
Locus tag A6J62_RS03390 Protein ID WP_000921691.1
Coordinates 753024..753545 (-) Length 174 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6J62_RS03350 749950..750363 - 414 WP_000049420.1 VOC family protein -
A6J62_RS03360 750547..751062 - 516 WP_000343779.1 GNAT family N-acetyltransferase -
A6J62_RS03365 751256..751540 + 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
A6J62_RS03370 751537..751815 + 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
A6J62_RS03375 751954..752349 - 396 WP_001000867.1 hypothetical protein -
A6J62_RS03385 752600..752725 - 126 WP_001905700.1 DUF645 family protein -
A6J62_RS03390 753024..753545 - 522 WP_000921691.1 DUF2442 domain-containing protein Antitoxin
A6J62_RS03395 753539..753808 - 270 WP_001114075.1 DUF4160 domain-containing protein Toxin
A6J62_RS03400 753839..753943 - 105 WP_099607150.1 acetyltransferase -
A6J62_RS03405 754000..754599 - 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
A6J62_RS03410 754749..755621 - 873 WP_000254716.1 DUF3800 domain-containing protein -
A6J62_RS03415 755796..756014 - 219 WP_071908318.1 GNAT family N-acetyltransferase -
A6J62_RS03420 756078..756515 - 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
A6J62_RS03425 756634..756843 - 210 Protein_647 GNAT family N-acetyltransferase -
A6J62_RS03430 756979..757368 - 390 WP_001081302.1 hypothetical protein -
A6J62_RS03435 757525..758442 - 918 WP_000186333.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 749087..881909 132822
inside Integron - - 749463..875103 125640
flank IS/Tn - - 756078..756515 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Protein of unknown function DUF4160

(9-74)

Antitoxin

Protein of unknown function DUF2442

(4-59)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10310.94 Da        Isoelectric Point: 6.6303

>T74615 WP_001114075.1 NZ_CP020407:c753808-753539 [Vibrio cholerae]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Download         Length: 270 bp

>T74615 NZ_CP020407:c753808-753539 [Vibrio cholerae]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin


Download         Length: 174 a.a.        Molecular weight: 19409.01 Da        Isoelectric Point: 6.7342

>AT74615 WP_000921691.1 NZ_CP020407:c753545-753024 [Vibrio cholerae]
MLKVIDVDFVSDHTLELTFNDGYQGYADLSVYFKKAPFSEIKDFKRFSLTRDGSLNWDGNELTAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Download         Length: 522 bp

>AT74615 NZ_CP020407:c753545-753024 [Vibrio cholerae]
ATGTTGAAAGTCATAGATGTTGATTTTGTCTCAGACCATACATTAGAACTGACGTTCAATGATGGTTACCAAGGCTATGC
CGACTTGTCGGTATACTTTAAAAAAGCACCGTTTTCAGAAATTAAGGACTTTAAACGCTTTTCCTTGACTCGTGATGGCT
CGTTAAATTGGGATGGTAATGAGTTAACGGCCGCGACACTCCGTGACATAACCAAGGGCTCACAAAAGTCGGTGGAGTTA
AGTTTTAATGTTCAAGAAATGGAAGCCGTCATCAAACAGGCCTCATGGGAATCGATGATGGAGGGGCGTCCCGACATCTT
GCAAGCGGCTATTCGTTCTTACGTAGAGCAGTTTGGTCATGGTCAAGTTATTGCAAAGGCGGGTATAAAAAGTCGTACGA
GTGCTTATCGCTCCTTGAAGCCAGAAACAACGCCAAACTTTGGCACACTAGTTCAGTTAGGGCACGCCGTCATCGAACTA
GCGAAAGATAGAACAGTGGCAAACAAGGAACCACGCATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086SLD6

Antitoxin

Source ID Structure
AlphaFold DB Q9KM84

References