Detailed information of TA system TA045812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 393467..394560
• Replicon: chromosome
• Accession: NZ_CP006948
• Organism: Vibrio cholerae O1 str. KW3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: N900_RS16015
• Protein ID: WP_000351248.1
• Coordinates: 394159..394560 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: N900_RS16010
• Protein ID: WP_001047169.1
• Coordinates: 393467..394159 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N900_RS15965	388751..389128	+	378	WP_000411109.1	hypothetical protein	-
N900_RS20370	389185..389299	+	115	Protein_394	acetyltransferase	-
N900_RS15970	389248..389529	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
N900_RS19650	389694..389807	+	114	WP_001889158.1	hypothetical protein	-
N900_RS19655	389756..389983	+	228	WP_073426587.1	DUF1289 domain-containing protein	-
N900_RS15980	390324..390656	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N900_RS15985	390643..390957	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
N900_RS15990	391094..391528	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
N900_RS20190	391696..391851	+	156	WP_000751734.1	hypothetical protein	-
N900_RS16000	391976..392956	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
N900_RS16005	393024..393296	+	273	Protein_403	CatB-related O-acetyltransferase	-
N900_RS16010	393467..394159	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
N900_RS16015	394159..394560	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
N900_RS20050	394530..394673	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
N900_RS16020	394748..395161	+	414	WP_000049417.1	VOC family protein	-
N900_RS16025	395375..395626	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
N900_RS16030	395616..395903	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N900_RS16035	396031..396486	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
N900_RS16040	396808..396960	+	153	WP_001884520.1	DUF645 family protein	-
N900_RS16045	397162..397659	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
N900_RS16050	397656..397928	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
N900_RS16055	398159..398827	+	669	WP_000043871.1	hypothetical protein	-
N900_RS16060	398979..399266	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304645..411088	106443
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	309725..410760	101035
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	391976..392956	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T45812 WP_000351248.1 NZ_CP006948:394159-394560 [Vibrio cholerae O1 str. KW3]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T45812 NZ_CP006948:394159-394560 [Vibrio cholerae O1 str. KW3]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT45812 WP_001047169.1 NZ_CP006948:393467-394159 [Vibrio cholerae O1 str. KW3]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT45812 NZ_CP006948:393467-394159 [Vibrio cholerae O1 str. KW3]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34