Detailed information of TA system TA058376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 119596..120211
• Replicon: chromosome
• Accession: NZ_CP013316
• Organism: Vibrio cholerae strain M2140

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: ASZ83_RS14600
• Protein ID: WP_001162670.1
• Coordinates: 119596..119883 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ83_RS14605
• Protein ID: WP_001232701.1
• Coordinates: 119894..120211 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ83_RS14555	115088..115345	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ83_RS14560	115333..115635	+	303	WP_000229318.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ83_RS14565	115924..116148	+	225	WP_001894423.1	DUF3709 domain-containing protein	-
ASZ83_RS14570	116592..116711	+	120	WP_071919600.1	DUF645 family protein	-
ASZ83_RS20010	116715..116768	+	54	Protein_123	DUF645 family protein	-
ASZ83_RS14585	117370..118374	+	1005	WP_000964931.1	LD-carboxypeptidase	-
ASZ83_RS14595	119149..119329	+	181	Protein_125	DUF645 family protein	-
ASZ83_RS14600	119596..119883	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ83_RS14605	119894..120211	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
ASZ83_RS20435	120208..120318	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
ASZ83_RS14615	120364..120903	+	540	WP_000099372.1	hypothetical protein	-
ASZ83_RS14620	121048..121482	+	435	WP_000453680.1	GNAT family N-acetyltransferase	-
ASZ83_RS14625	121709..121987	+	279	WP_001921601.1	DUF3709 domain-containing protein	-
ASZ83_RS20030	122239..122445	+	207	Protein_132	DUF3709 domain-containing protein	-
ASZ83_RS20035	122645..122746	+	102	WP_001921603.1	hypothetical protein	-
ASZ83_RS14630	122736..123122	+	387	WP_000703163.1	VOC family protein	-
ASZ83_RS14635	123317..123568	+	252	WP_001890111.1	TIGR03643 family protein	-
ASZ83_RS14640	123541..123690	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
ASZ83_RS14645	123804..124247	+	444	WP_000803902.1	hypothetical protein	-
ASZ83_RS14650	124251..124361	+	111	WP_071908336.1	DUF3265 domain-containing protein	-
ASZ83_RS14655	124574..124726	+	153	WP_001884520.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	87981..224211	136230
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.008	inside	Integron	-	-	93061..221594	128533

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T58376 WP_001162670.1 NZ_CP013316:119596-119883 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T58376 NZ_CP013316:119596-119883 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT58376 WP_001232701.1 NZ_CP013316:119894-120211 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT58376 NZ_CP013316:119894-120211 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36