Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 363251..363866 Replicon chromosome
Accession NZ_CP046841
Organism Vibrio cholerae strain F9993

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag GPY07_RS16120 Protein ID WP_001162670.1
Coordinates 363579..363866 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GPY07_RS16115 Protein ID WP_001232701.1
Coordinates 363251..363568 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPY07_RS16070 358660..359175 - 516 WP_001201528.1 lipocalin family protein -
GPY07_RS16075 359312..359788 - 477 WP_001047180.1 GNAT family N-acetyltransferase -
GPY07_RS16080 359816..359926 - 111 WP_071917832.1 DUF3265 domain-containing protein -
GPY07_RS16085 359923..360852 - 930 WP_000335802.1 hypothetical protein -
GPY07_RS16090 360972..361418 - 447 WP_000128977.1 hypothetical protein -
GPY07_RS16095 361571..362575 - 1005 WP_000964925.1 LD-carboxypeptidase -
GPY07_RS16100 362788..362826 - 39 WP_106019119.1 hypothetical protein -
GPY07_RS16105 362842..362967 - 126 WP_001944767.1 DUF645 family protein -
GPY07_RS19445 363144..363254 - 111 WP_134814058.1 DUF3265 domain-containing protein -
GPY07_RS16115 363251..363568 - 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
GPY07_RS16120 363579..363866 - 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GPY07_RS16125 364133..364313 - 181 Protein_353 DUF645 family protein -
GPY07_RS16130 364738..364971 - 234 WP_032482776.1 DUF3709 domain-containing protein -
GPY07_RS16135 365374..365592 - 219 WP_001917086.1 DUF3709 domain-containing protein -
GPY07_RS16140 365902..366555 - 654 WP_000226874.1 hypothetical protein -
GPY07_RS16145 366612..367121 - 510 WP_000405987.1 GNAT family N-acetyltransferase -
GPY07_RS16150 367363..367605 + 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GPY07_RS16155 367624..367941 + 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
GPY07_RS19450 368135..368230 - 96 WP_001907607.1 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 322056..419024 96968
inside Integron - - 322432..412236 89804


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T145066 WP_001162670.1 NZ_CP046841:c363866-363579 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T145066 NZ_CP062252:5678900-5679178 [Pseudomonas allokribbensis]
ATGATCAAATCCTTTCAGCACAAAGGCCTTCGTGGCTTCTATGAAACCGGTTCGACTCGCGGGATTCGCGCCGATCACGC
TAAACGGCTGTCGCGTATGCTGCAGTTCATGGATCGGGCAGCGGTTCCCGGGGATCTGGACCTTCCCGGTTGGCGTCTGC
ATCCACTGAAAGGGGAACTTTGCGAGTACTGGTCACTGAGCGTGTCCGGGAACTGGCGGGTTATCTTTCGATTTGCAGGG
TCGGACATCGAACTGGTTGATTATCTGGATTACCACTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT145066 WP_001232701.1 NZ_CP046841:c363568-363251 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT145066 NZ_CP062252:5679193-5679480 [Pseudomonas allokribbensis]
ATGCCCATGCACAACCCGCCACACCCCGGTGAAACGCTGCTGATGGATGTATTGCCGGAGCTTGGTATCAGTGTCACCGA
ATTGGCTCGGCATCTCGGATTCGCCCGCCCTCACCTTTCTCGTGTATTGCACGGACATGCGCCGATCAGCCCGGATCTGG
CCGTACGACTGGAGCGCGCGGGGATTGGCAAGGCTCGTATGTGGCTAGGCGTTCAAACCGATTACGACCTTTGGCAAGCC
GAGCACCGCGAGCAGCCAGCCATTCAACCAATCGCTGCTCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References