Detailed information of TA system TA145813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 415351..415984
• Replicon: chromosome
• Accession: NZ_CP047296
• Organism: Vibrio cholerae C6706

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: GTF72_RS15990
• Protein ID: WP_000843587.1
• Coordinates: 415351..415683 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTF72_RS15995
• Protein ID: WP_000071008.1
• Coordinates: 415670..415984 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF72_RS15925	410792..410995	+	204	WP_001911745.1	hypothetical protein	-
GTF72_RS15930	411276..411449	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
GTF72_RS15935	411809..412078	+	270	WP_001198131.1	hypothetical protein	-
GTF72_RS15940	412389..412475	+	87	WP_134820423.1	DUF645 family protein	-
GTF72_RS15945	412475..412567	+	93	Protein_437	DUF645 family protein	-
GTF72_RS15950	412776..413162	+	387	WP_000703163.1	VOC family protein	-
GTF72_RS15955	413364..413606	+	243	WP_000107461.1	hypothetical protein	-
GTF72_RS15960	413777..414154	+	378	WP_000411109.1	hypothetical protein	-
GTF72_RS15965	414334..414555	+	222	WP_032468461.1	DUF1289 domain-containing protein	-
GTF72_RS15970	414531..414689	-	159	WP_001894455.1	DUF1196 family protein	-
GTF72_RS15975	414721..414834	+	114	WP_001889158.1	hypothetical protein	-
GTF72_RS15980	414783..415010	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
GTF72_RS15985	415040..415198	-	159	WP_001882309.1	DUF1196 family protein	-
GTF72_RS15990	415351..415683	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTF72_RS15995	415670..415984	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
GTF72_RS16000	416121..416555	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
GTF72_RS16005	416723..416878	+	156	WP_000751734.1	hypothetical protein	-
GTF72_RS16010	417003..417983	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
GTF72_RS16015	418051..418323	+	273	Protein_451	CatB-related O-acetyltransferase	-
GTF72_RS16020	418494..419186	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
GTF72_RS16025	419186..419587	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
GTF72_RS16030	419557..419700	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
GTF72_RS16035	419775..420188	+	414	WP_000049417.1	VOC family protein	-
GTF72_RS16040	420402..420653	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GTF72_RS16045	420643..420930	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306905..436165	129260
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	311985..435789	123804
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	417003..417983	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T145813 WP_000843587.1 NZ_CP047296:415351-415683 [Vibrio cholerae C6706]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T145813 NZ_CP062700:2765581-2765757 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT145813 WP_000071008.1 NZ_CP047296:415670-415984 [Vibrio cholerae C6706]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT145813 NZ_CP062700:2765803-2766219 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA