Detailed information of TA system TA038623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 71593..72208
• Replicon: chromosome
• Accession: NZ_AP024548
• Organism: Vibrio cholerae strain BCH-01536

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: K6J77_RS14425
• Protein ID: WP_001162670.1
• Coordinates: 71593..71880 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J77_RS14430
• Protein ID: WP_001232701.1
• Coordinates: 71891..72208 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J77_RS19030	66936..67111	+	176	Protein_122	acetyltransferase	-
K6J77_RS19035	67172..67324	+	153	Protein_123	DUF645 family protein	-
K6J77_RS14390	67518..67835	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K6J77_RS14395	67854..68096	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K6J77_RS14400	68338..68847	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
K6J77_RS14405	68904..69557	+	654	WP_000226874.1	hypothetical protein	-
K6J77_RS19040	69646..69855	+	210	WP_001900246.1	DUF3709 domain-containing protein	-
K6J77_RS14410	69867..70085	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
K6J77_RS19045	70274..70467	+	194	Protein_130	hypothetical protein	-
K6J77_RS14415	70488..70721	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
K6J77_RS14420	71146..71326	+	181	Protein_132	DUF645 family protein	-
K6J77_RS19050	71439..71552	+	114	WP_001894955.1	hypothetical protein	-
K6J77_RS14425	71593..71880	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J77_RS14430	71891..72208	+	318	WP_001232701.1	HigA family addiction module antitoxin	Antitoxin
K6J77_RS19055	72205..72315	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
K6J77_RS19060	72354..72464	+	111	Protein_137	acetyltransferase	-
K6J77_RS14435	72492..72617	+	126	WP_001944767.1	DUF645 family protein	-
K6J77_RS19065	72580..72696	+	117	WP_001889170.1	hypothetical protein	-
K6J77_RS14445	72884..73888	+	1005	WP_000964925.1	LD-carboxypeptidase	-
K6J77_RS14450	74041..74487	+	447	WP_000128977.1	hypothetical protein	-
K6J77_RS14455	74607..75536	+	930	WP_000335802.1	hypothetical protein	-
K6J77_RS19070	75533..75643	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
K6J77_RS14460	75671..76147	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
K6J77_RS14465	76338..76799	+	462	WP_001911752.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	11978..113648	101670

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T38623 WP_001162670.1 NZ_AP024548:71593-71880 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T38623 NZ_AP024548:71593-71880 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT38623 WP_001232701.1 NZ_AP024548:71891-72208 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT38623 NZ_AP024548:71891-72208 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36