Detailed information of TA system TA058227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 448472..449105
• Replicon: chromosome
• Accession: NZ_CP013302
• Organism: Vibrio cholerae strain C5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: ASZ80_RS16935
• Protein ID: WP_000843587.1
• Coordinates: 448472..448804 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ80_RS16940
• Protein ID: WP_000071008.1
• Coordinates: 448791..449105 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ80_RS20880	443913..444116	+	204	WP_001911745.1	hypothetical protein	-
ASZ80_RS20675	444397..444570	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
ASZ80_RS16875	444930..445199	+	270	WP_001198131.1	hypothetical protein	-
ASZ80_RS21045	445517..445688	+	172	Protein_489	DUF645 family protein	-
ASZ80_RS16890	445897..446283	+	387	WP_000703163.1	VOC family protein	-
ASZ80_RS16895	446485..446727	+	243	WP_000107461.1	hypothetical protein	-
ASZ80_RS16900	446898..447275	+	378	WP_000411109.1	hypothetical protein	-
ASZ80_RS21010	447332..447446	+	115	Protein_493	acetyltransferase	-
ASZ80_RS16905	447395..447676	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
ASZ80_RS16920	447842..447955	+	114	WP_001889158.1	hypothetical protein	-
ASZ80_RS16925	447904..448131	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
ASZ80_RS16935	448472..448804	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ80_RS16940	448791..449105	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
ASZ80_RS16945	449242..449676	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
ASZ80_RS20780	449844..449999	+	156	WP_000751734.1	hypothetical protein	-
ASZ80_RS16960	450124..451104	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
ASZ80_RS16965	451172..451478	+	307	Protein_502	CatB-related O-acetyltransferase	-
ASZ80_RS16970	451615..452307	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
ASZ80_RS16975	452307..452708	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
ASZ80_RS20690	452678..452821	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ80_RS16985	452896..453309	+	414	WP_000049417.1	VOC family protein	-
ASZ80_RS16990	453523..453774	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ80_RS16995	453764..454051	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304694..469286	164592
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309774..468910	159136
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	450124..451104	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T58227 WP_000843587.1 NZ_CP013302:448472-448804 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T58227 NZ_CP013302:448472-448804 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT58227 WP_000071008.1 NZ_CP013302:448791-449105 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT58227 NZ_CP013302:448791-449105 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA