Detailed information of TA system TA025715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 118633..119726
• Replicon: chromosome
• Accession: NC_016446
• Organism: Vibrio cholerae O1 str. 2010EL-1786

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: HJ37_RS14790
• Protein ID: WP_000351248.1
• Coordinates: 119325..119726 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: HJ37_RS14785
• Protein ID: WP_001047169.1
• Coordinates: 118633..119325 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ37_RS14745	113916..114293	+	378	WP_000411109.1	hypothetical protein	-
HJ37_RS20235	114350..114464	+	115	Protein_173	acetyltransferase	-
HJ37_RS19565	114413..114694	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
HJ37_RS19580	114860..114973	+	114	WP_001889158.1	hypothetical protein	-
HJ37_RS19585	114922..115149	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
HJ37_RS14755	115490..115822	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HJ37_RS14760	115809..116123	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
HJ37_RS14765	116260..116694	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
HJ37_RS20240	116862..117017	+	156	WP_000751734.1	hypothetical protein	-
HJ37_RS14775	117142..118122	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
HJ37_RS14780	118190..118462	+	273	Protein_182	CatB-related O-acetyltransferase	-
HJ37_RS14785	118633..119325	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
HJ37_RS14790	119325..119726	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HJ37_RS20025	119696..119839	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
HJ37_RS14795	119914..120327	+	414	WP_000049417.1	VOC family protein	-
HJ37_RS14800	120541..120792	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HJ37_RS14805	120782..121069	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HJ37_RS14810	121197..121652	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
HJ37_RS14815	121974..122126	+	153	WP_001884520.1	DUF645 family protein	-
HJ37_RS14820	122328..122825	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
HJ37_RS14825	122822..123094	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
HJ37_RS14830	123325..123993	+	669	WP_000043871.1	hypothetical protein	-
HJ37_RS14835	124145..124432	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31115..136304	105189
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	catB9	-	36195..135928	99733
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	117142..118122	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T25715 WP_000351248.1 NC_016446:119325-119726 [Vibrio cholerae O1 str. 2010EL-1786]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T25715 NC_016446:119325-119726 [Vibrio cholerae O1 str. 2010EL-1786]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT25715 WP_001047169.1 NC_016446:118633-119325 [Vibrio cholerae O1 str. 2010EL-1786]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT25715 NC_016446:118633-119325 [Vibrio cholerae O1 str. 2010EL-1786]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34